Fatty acid profiles of M. menidia females and their unfertilized eggs.

Abstract

Gas chromatography was used to quantify the absolute (mg g dry weight-1) and relative concentrations (% of total) of 27 FAs for each of 5 females (whole individual) and their unfertilized eggs (~ 1 ml). Samples were first dried and then homogenized in a solution of chloroform-methanol (2:1 v/v) and tricosanoic acid (23:0) as an internal standard for quantification of mg g−1 dry mass of fatty acids. Lipids were cold-extracted from approximately 50 mg of dry mass. A Shimadzu GC-2014 gas chromatograph set with a Phenomenex ZB-WAX plus capillary column (30 m long; 0.53 mm ID; 1.0 µm thick) was used to quantify FAs, and individual FAs were identified by comparison to commercial standards (Supelco, Inc). These data are published in "Snyder, J.T.*, Murray, C.S.*, and Baumann, H. (2017) Potential for maternal effects on offspring CO2-sensitivities in a coastal marine fish. Journal of Experimental Marine Biology and Ecology. doi: 10.1016/j.jembe.2017.11.002” and are affiliated with the dataset "Survival, length, and growth responses of M. menidia offspring from different females exposed to contrasting CO2 environments. doi: XXXXX" For a complete list of measurements, refer to the supplemental document 'Field_names.pdf', and a full dataset description is included in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: http://www.bco-dmo.org/dataset/719379