Biological and Chemical Oceanography Data Management Office Data Sets
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The Biological and Chemical Oceanography Data Management Office (BCO-DMO) was created to serve PIs funded by the NSF Biological and Chemical Oceanography Sections as a location where marine biogeochemical, ecological and oceanographic data and information developed in the course of scientific research can easily be disseminated, protected, and stored on short and intermediate time-frames. Our main objective is to support the scientific community through improved access to ocean science data.
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DatasetWeekly surface water samples from Newport Pier, CA collected between 11 January 2012 and 3 November 2023(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-03-05)Weekly surface water samples from Newport Pier, CA, were collected between 11 January 2012 and 3 November 2023. This project is a continuation of a time series with weekly surface water sampling from the Microbes in the Coastal Region of Orange County (MICRO) time series station at Newport Pier (33°36.37’N, 117°55.87’W). We measured dissolved nitrate and phosphate in the water, as well as particulate organic carbon, nitrogen and phosphorus. These data were published in Fagan et al. (2019), Larkin et al. (2020), and a pending publication, Larkin et al. (in press). For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/564351
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DatasetWeather Station data from University of Washington Friday Harbor Laboratories, Friday Harbor WA, Cantilever Point from 2006 to 2024(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-03-04)This dataset includes parameters measured by a weather station at the University of Washington Friday Harbor Laboratories, Friday Harbor WA, Cantilever Point from 2006 to 2024. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/491262
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DatasetDissolved trace metal and macronutrient concentrations from field samples collected during the EXPORTS North Atlantic campaign at the Porcupine Abyssal Plain-Sustained Observatory (PAP-SO) site on board the RRS Discovery (DY131) in May 2021(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-03-03)This dataset includes dissolved trace metal (manganese, iron, cobalt, nickel, copper, cadmium, zinc, lead) and macronutrient (nitrate+nitrite, phosphate, silicic acid, nitrite) concentration data from field samples collected during the EXPORTS North Atlantic campaign at the Porcupine Abyssal Plain-Sustained Observatory (PAP-SO) site on board the RRS Discovery (DY131). These data were primarily collected opportunistically during the course of water collection for incubation experiments (see separate dataset for incubations), and provide field context for the shipboard incubation experiments as well as temporal characterization of a retentive anticyclonic eddy occupied over the course of the cruise. This research focuses on the vertical export of the carbon associated with a major group of phytoplankton, the diatoms in the North Atlantic near the Porcupine Abyssal Plain. The major objective is to understand how diatom community composition and the prevailing nutrient conditions create taxonomic differences in metabolic state that combine to direct diatom taxa to different carbon export pathways. The focus is on diatoms, given their large contribution to global marine primary productivity and carbon export which translates into a significant contribution to the biogeochemical cycling of carbon (C), nitrogen (N), phosphorus (P), iron (Fe) and silicon (Si). It is hypothesized that the type and degree of diatom physiological stress are vital aspects of ecosystem state that drive export. To test this hypothesis, combined investigator expertise in phytoplankton physiology, genomics, and trace element chemistry is used to assess the rates of nutrient use and the genetic composition and response of diatom communities, with measurements of silicon and iron stress to evaluate stress as a predictor of the path of diatom carbon export. The EXPORTS field campaign in the North Atlantic sampled a retentive eddy over nearly a month in May 2021, which coincided with the decline of the North Atlantic Spring Bloom. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/954941
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DatasetCOI mtDNA sequences for trematodes from fish collections across the Northern Line Islands and French Polynesia archipelagos collected between 2009 and 2023(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-03-03)The loss of biological diversity is considered one of the principal environmental challenges of the 21st century, and there are hints that this massive reorganization of food webs could affect how parasites are transmitted among hosts. Parasites are often hidden and can be easy to overlook, but they are ecologically important and ubiquitous - so it is important to understand whether we should expect more or fewer of them as biodiversity disappears. Does biodiversity loss increase the abundance of parasites by eroding natural "checks and balances" on transmission? Or does it decrease parasite abundance by removing the free-living biodiversity on which parasites depend? Answers to these questions are urgently needed if we are to mitigate or prevent an uptick in parasite transmission for ecosystems experiencing biodiversity loss. In a joint collaborative research project among the University of Washington, Scripps Institution of Oceanography at UC San Diego, and California State University Monterey Bay, we created a parasite dataset of unprecedented size and taxonomic resolution. We sampled parasites of coral reef fishes from 19 replicate islands in the central equatorial Pacific to study how biodiversity and parasite burden covary. This data set contains COI mtDNA sequence accession numbers, collection locations, and life stages for trematodes from the family Microscaphidiidae and Paramphistomatidae from fish collections across the Northern Line Islands and French Polynesia archipelagos collected between 2009 and 2023. Specifically this data set represents 87 Microscaphidiidae samples from the Northern Line Islands and 132 Paramphistomatidae from French Polynesia. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/953401
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DatasetBacteria Counts CTD Bottle Measurements from CTD samples collected during R/V Hugh R. Sharp cruise HRS2204 from Apr to May 2022(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-02-28)These data include measurements of free bacteria in whole seawater and particle-associated bacteria for particles larger than 1.2 microns from CTD bottle water samples collected during a cruise on the Northeast Continental Shelf to study particle disaggregation. One cruise was completed aboard the R/V Hugh R. Sharp from 2022-04-21 through 2022-05-02 (HRS 22-04), which visited a variety of stations and hydrodynamic environments associated with the Northeast Continental Shelf of the United States. Stations ranged from Georges Bank and the Great South Channel near the Gulf of Maine, Martha's Vineyard, the mouth of the Sakonnet River near Newport, Rhode Island, and Hudson Canyon near New York. These data were collected as part of a study to clarify the importance of hydrodynamic forces on the cohesion, aggregation, and breakup of marine particles. These data were collected by Dr. Austin Grubb of the Rutgers University on the cruise led by Dr. Matthew Rau (chief scientist) of the George Washington University. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/945987
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DatasetGene expression of Pocillopora damicornis collected from reef of Heron Island, southern Great Barrier Reef from Jan 2021 to Feb 2021(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-02-28)Corals residing in habitats that experience frequent seawater pCO2 variability may possess an enhanced capacity to cope with ocean acidification. Yet, we lack a clear understanding of the molecular toolkit enabling acclimatization to environmental extremes, and how life-long exposure to pCO2 variability influences biomineralization. We examined the gene expression responses and micro-skeletal characteristics of Pocillopora damicornis originating from the reef flat and reef slope of Heron Island, southern Great Barrier Reef. The reef flat (454 ± 3.0) and reef slope (418 ± 1.9) had similar mean seawater pCO2 (µatm; mean ± SE), but the reef flat experienced twice the mean daily pCO2 amplitude (range of 797v. 399 µatm day-1, respectively). A controlled mesocosm experiment was conducted over eight weeks, from mid-January to late-March 2021, exposing P. damicornis from the reef slope and reef flat to stable (218±9) or variable (911±31) diel pCO2 fluctuations (µatm; mean ± SE). This dataset includes the gene expression data for these samples. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/942938
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DatasetTurbulence microstructure profiles collected during R/V Hugh R. Sharp cruise HRS2204 from Apr to May 2022(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-02-27)These data include profiles of turbulence dissipation rate obtained from a Rockland Scientific VMP-250 deployed during a cruise on the Northeast Continental Shelf to study particle disaggregation. One cruise was completed aboard the R/V Hugh R. Sharp from 2022-04-21 through 2022-05-02 (HRS22-04), which visited a variety of stations and hydrodynamic environments associated with the Northeast Continental Shelf of the United States. Cruise stations where dissipation rate profiles were obtained ranged from Georges Bank and the Great South Channel near the Gulf of Maine, and Hudson Canyon near New York. These data were collected as part of a study to clarify the importance of hydrodynamic forces on the cohesion, aggregation, and breakup of marine particles. These data were collected by Dr. Matthew Rau (chief scientist) of the George Washington University. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/945981
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DatasetBiogenic Silica from CTD samples collected during R/V Hugh R. Sharp cruise HRS2204 from Apr to May 2022(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-02-27)These data include measurements of biogenic silica from CTD bottle water samples collected during a cruise on the Northeast Continental Shelf to study particle disaggregation. One cruise was completed aboard the R/V Hugh R. Sharp from 2022-04-21 through 2022-05-02 (HRS22-04), which visited a variety of stations and hydrodynamic environments associated with the Northeast Continental Shelf of the United States. Stations ranged from Georges Bank and the Great South Channel near the Gulf of Maine, Martha's Vineyard, the mouth of the Sakonnet River near Newport, Rhode Island, and Hudson Canyon near New York. These data were collected as part of a study to clarify the importance of hydrodynamic forces on the cohesion, aggregation, and breakup of marine particles. These data were collected by Dr. Austin Grubb of the Rutgers University on the cruise led by Dr. Matthew Rau (chief-sci) of the George Washington University. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/945973
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DatasetTransparent Exopolymer Particles (TEP) from CTD samples collected during R/V Hugh R. Sharp cruise HRS2204 from Apr to May 2022(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-02-27)These data include measurements of transparent exopolymer particles (TEP) from CTD bottle water samples collected during a cruise on the Northeast Continental Shelf to study particle disaggregation. One cruise was completed aboard the R/V Hugh R. Sharp from 2022-04-21 through 2022-05-02 (HRS22-04), which visited a variety of stations and hydrodynamic environments associated with the Northeast Continental Shelf of the United States. Stations ranged from Georges Bank and the Great South Channel near the Gulf of Maine, Martha's Vineyard, the mouth of the Sakonnet River near Newport, Rhode Island, and Hudson Canyon near New York. TEP measurements were performed following recently published methods relying on Alcian Blue staining protocols and spectrophotometry. These data were collected as part of a study to clarify the importance of hydrodynamic forces on the cohesion, aggregation, and breakup of marine particles. These data were collected by Dr. Kieran Curran of the University of New Hampshire on the cruise led by Dr. Matthew Rau (chief-sci) of the George Washington University. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/945964
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DatasetDark DIC Fixation Rates collected from CliOMZ AT50-10 in the Eastern Pacific Ocean from May to June 2023 (CliOMZ project)(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-02-27)These data include dark dissolved inorganic carbon (DIC) fixation rates measured on R/V Atlantis (CliOMZ AT50-10 expedition) from Golfito, Costa Rica to San Diego, USA in May-June 2023. We aimed at quantifying dark DIC fixation rates associated with nitrification by specifically inhibiting ammonia-oxidizing microorganisms. Instruments used were a CTD profiler and a scintillation counter (Perkin-Elmer Tri-Carb 2910 TR). For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/948396
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DatasetHeterotrophic Production Rates collected from CliOMZ AT50-10 in the Eastern Pacific Ocean from May to June 2023 (CliOMZ project)(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-02-27)These data include heterotrophic production rates measured on R/V Atlantis (CliOMZ AT50-10 expedition) from Golfito, Costa Rica to San Diego, USA in May-June 2023. We aimed at comparing heterotrophic and chemoautotrophic microbial activities at the selected study sites. Instruments used were a CTD profiler and a scintillation counter (Perkin-Elmer Tri-Carb 2910 TR). For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/948411
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DatasetOOI Global Irminger Sea Array CTD and Discrete Water Sampling Data from Mooring Overturning Cruises in the Irminger Sea from 2014-2023 (OOI Cruise Data project)(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-02-27)The hydrographic sampling performed by The NSF Ocean Observatories Initiative Coastal and Global Scale Nodes(OOI CGSN) as part of each Array turn represents a significant collection of valuable physical, chemical, and biological information. In addition to the CTD, collected hydrographic data include discrete oxygen, salinity, nutrient (nitrate, nitrite, silicate, phosphate, ammonium), chlorophyll, and carbon system measurements. These data serve several important functions. First, they are necessary for the calibration and evaluation of the moored instrumentation at each Array. Furthermore, the annual (Global) or biannual (Coastal) collection of data at the same locations provides a unique timeseries of a large set of water properties following established community standards and methods, independent of its association with the OOI moorings. The analysis of collected water samples for the parameters listed above are performed by a number of outside labs on behalf of OOI-CGSN. Consequently, the water sampling data for a given cruise is distributed among a number of different files. The Discrete Sampling Summary integrates the related CTD, metadata, and discrete water sample data into a single file. Additionally, it synthesizes qualitative and quantitative information about the quality of a measurement into data quality flags for each associated parameter which follow WOCE-standards. The final product is the Discrete Sampling Summary spreadsheet which contains the metadata, CTD data, and discrete water sample data into a single spreadsheet with data quality flags. This dataset includes hydrographic data from the Global Irminger Sea Array located in the North Atlantic to the southeast of Greenland.This region experiences high winds and large surface waves, strong atmosphere-ocean exchanges of energy and gases, carbon dioxide sequestration, high biological productivity, and an important fishery. It is one of the few places on Earth with deep-water formation that feeds the large-scale thermohaline circulation. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/911407
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DatasetOOI Global Station Papa Array CTD and Discrete Water Sampling Data from Mooring Overturning Cruises in the Gulf of Alaska from 2013 to 2023 (OOI Cruise Data project)(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-02-27)The hydrographic sampling performed by OOI-CGSN (the Ocean Observatories Initiative - Coastal and Global Scale Nodes) as part of each Array turn represents a significant collection of valuable physical, chemical, and biological information. In addition to the CTD, collected hydrographic data include discrete oxygen, salinity, nutrient (nitrate, nitrite, silicate, phosphate, ammonium), chlorophyll, and carbon system measurements. These data serve several important functions. First, they are necessary for the calibration and evaluation of the moored instrumentation at each Array. Furthermore, the annual (Global) or biannual (Coastal) collection of data at the same locations provides a unique time series of a large set of water properties following established community standards and methods, independent of its association with the OOI moorings. The analyses of collected water samples for the parameters listed above are performed by a number of outside labs on behalf of OOI-CGSN. Consequently, the water sampling data for a given cruise is distributed among a number of different files. The Discrete Sampling Summary integrates the related CTD, metadata, and discrete water sample data into a single file. Additionally, it synthesizes qualitative and quantitative information about the quality of a measurement into data quality flags for each associated parameter which follow WOCE-standards. The final product is the Discrete Sampling Summary spreadsheet which contains the metadata, CTD data and discrete water sample data into a single spreadsheet with data quality flags. This dataset includes hydrographic data from the Global Station Papa Array located in the Gulf of Alaska next to the NOAA Pacific Marine Environmental Laboratory (PMEL) Surface Buoy. The region is extremely vulnerable to ocean acidification, has a productive fishery, and low eddy variability. It is impacted by the Pacific Decadal Oscillation and adds to a broader suite of OOI and other observatory sites in the Northeast Pacific. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/923122
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DatasetSalp and pteropod associated microorganisms from the Western Edge of the Gulf Stream sampled in September 2019.(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-02-27)Microbial mortality impacts the structure of food webs, carbon flow, and the interactions that create dynamic patterns of abundance across gradients in space and time in diverse ecosystems. In the oceans, estimates of microbial mortality by viruses, protists, and small zooplankton do not account fully for observations of loss, suggesting the existence of underappreciated mortality sources. We examined how ubiquitous mucous mesh feeders (i.e. gelatinous zooplankton) could contribute to microbial mortality in the open ocean. We coupled capture of live animals by blue-water diving to sequence-based approaches to measure the enrichment and selectivity of feeding by two coexisting mucous grazer taxa (pteropods and salps) on numerically dominant marine prokaryotes. We show that mucous mesh grazers consume a variety of marine prokaryotes and select between coexisting lineages and similar cell sizes. We show that Prochlorococcus may evade filtration more than other cells and that planktonic archaea are consumed by macrozooplanktonic grazers. Discovery of these feeding relationships identifies a new source of mortality for Earth's dominant marine microbes and alters our understanding of how top-down processes shape microbial community and function. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/926841
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DatasetEukaryotic and prokaryotic microbial taxa retained by wild-caught doliolids collected during bloom events at three different shelf locations in the northern California Current system in June 2019.(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-02-27)Doliolids have a unique ability to impact the marine microbial community through bloom events and high filtration rates. Their predation on large eukaryotic microorganisms is established and evidence of predation on smaller prokaryotic microorganisms is beginning to emerge. We studied the retention of both eukaryotic and prokaryotic microbial taxa by wild-caught doliolids in the northern California Current system. Doliolids were collected during bloom events identified at three different shelf locations with variable upwelling intensity. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/926299
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Dataset16S rRNA gene of microorganisms sampled along the Newport Hydrographic (NH) and Trinidad Head (TR) lines, in OR and CA in 2018 and 2019(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-02-27)The Northern California Current ecosystem is a productive system which supports major fisheries. To determine how the microbial community responds to variable upwelling, we examined the 16S rRNA gene of microorganisms from two size fractions, 0.2-1.6µm and greater than 1.6µm along the Newport Hydrographic (NH) and Trinidad Head (TR) lines, in OR and CA. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/926850
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DatasetHigh-throughput sequencing of the 16S rRNA gene, microscopy, and flow cytometry of pyrosome-associated microorganisms compared to seawater sampled during a Pyrosoma atlanticum bloom in the Northern California Current System in July 2018.(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-02-27)Pyrosomes are widely distributed pelagic tunicates that have the potential to reshape marine food webs when they bloom. However, their grazing preferences and interactions with the background microbial community are poorly understood. The diversity, relative abundance, and taxonomy of pyrosome-associated microorganisms were compared to seawater during a Pyrosoma atlanticum bloom in the Northern California Current System using high-throughput sequencing of the 16S rRNA gene, microscopy, and flow cytometry. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/926093
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DatasetMeteorological observations from NOAA station 8761724, Grande Isle, LA from September 20, 2022, to September 29, 2024.(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-02-24)Subset of the NOAA Tides and Currents NOAA webpage. It includes meteorological observations of wind speed and direction which, in conjunction with landscape data, are used as a rough calculation of fetch distance in this study. Ultimately, the fetch distance variable was not included in the final analysis. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/953856
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DatasetTime series of T. suecica densities under fluctuating temperatures experiment from May 2023 to Aug 2023(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-02-24)This dataset includes the densities (count/mL) of the alga Tetraselmis suecica (LB 2286) cultures grown from a UTEX sample. The counts were obtained using a Beckman Coulter Z2 Particle Counter in the lab over a five-day period under different experimental temperature regimes. The experiment took place between 2023-05-23 and 2023-08-18. The goal of the experiment was to determine how mean temperature and temperature fluctuation frequency affected the growth rate of T. suecica. By comparing the growth rate of T. suecica under constant vs. variable temperatures, one can determine the historical or legacy effects of past temperature variation on subsequent organismal performance. These experiments were conducted by members of the Brian Helmuth Lab at Northeastern University. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/953492
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DatasetDepth profile chlorophyll, macronutrient, trace metal, metal speciation, and carbon system data from FeOA project cruise SKQ202209S on the R/V Sikuliaq in the Northeast Pacific between June and July 2022(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2025-02-21)This dataset includes the concentrations of dissolved inorganic macronutrients (phosphate, nitrate plus nitrite (N+N), silicic acid, and nitrite), chlorophyll a and phaeophytin, dissolved trace metals (iron, manganese, nickel, zinc, copper), and labile dissolved nickel, as well as pH and total alkalinity measurements, from discrete depth profile samples collected on the FeOA cruise SKQ202209S on R/V Sikuliaq in the Northeast Pacific from June to July 2022. This project investigates the effects of ocean acidification on the associations between iron and organic ligands in seawater and on iron bioavailability to marine phytoplankton communities. The project used a combination of shipboard incubation experiments and depth profiles to characterize iron speciation and cycling across coastal upwelling, oligotrophic open ocean, and iron-limited subarctic oceanographic regimes in the NE Pacific. Surface seawater was incubated at pH of 8.1, 7.6, and 7.1 with natural iron and with dissolved iron amendments in order to investigate interactions between pH and iron bioavailability across the different regimes. Understanding how pH influences iron and its relationship with ligands provides important information for assessing the impacts of ocean acidification on primary production and biogeochemical processes. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/950296