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The Marine Biological Laboratory is an international center for research, education, and training in biology, biomedicine, and ecology.
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Dynamic organization of chromatin domains revealed by super-resolution live-dell imaging
(2017-06)The eukaryotic genome is organized within cells as chromatin. For proper information output, higher-order chromatin structures can be regulated dynamically. How such structures form and behave in various cellular processes ... -
Grasshopper DCMD : an undergraduate electrophysiology lab for investigating single-unit responses to behaviorally-relevant stimuli
(Faculty of Undergraduate Neuroscience, 2017-05)Avoiding capture from a fast-approaching predator is an important survival skill shared by many animals. Investigating the neural circuits that give rise to this escape behavior can provide a tractable demonstration of ... -
Protein arginine methyltransferases interact with intraflagellar transport particles and change location during flagellar growth and resorption
(American Society for Celll Biology, 2017-03-15)Changes in protein by posttranslational modifications comprise an important mechanism for the control of many cellular processes. Several flagellar proteins are methylated on arginine residues during flagellar resorption; ... -
Expanding the genetic toolkit in Xenopus : approaches and opportunities for human disease modeling
(Elsevier, 2016-04-22)The amphibian model Xenopus, has been used extensively over the past century to study multiple aspects of cell and developmental biology. Xenopus offers advantages of a non-mammalian system, including high fecundity, ... -
Dramatic fighting by male cuttlefish for a female mate
(University of Chicago Press, 2017-05-02)Male cuttlefish compete for females with a repertoire of visually dramatic behaviors. Laboratory experiments have explored this system in Sepia officinalis, but corroborative field data have eluded collection attempts by ... -
Characterization of trace metal content in the developing zebrafish embryo
(Public Library of Science, 2017-06-15)Trace metals are essential for health but toxic when present in excess. The maintenance of trace metals at physiologic levels reflects both import and export by cells and absorption and excretion by organs. The mechanism ... -
Leaching losses from Kenyan maize cropland receiving different rates of nitrogen fertilizer
(Springer, 2017-05-16)Meeting food security requirements in sub-Saharan Africa (SSA) will require increasing fertilizer use to improve crop yields, however excess fertilization can cause environmental and public health problems in surface and ... -
Bacterial biogeography across the Amazon river-ocean continuum
(Frontiers Media, 2017-05-23)Spatial and temporal patterns in microbial biodiversity across the Amazon river-ocean continuum were investigated along ∼675 km of the lower Amazon River mainstem, in the Tapajós River tributary, and in the plume and coastal ... -
Visualizing calcium flux in freely moving nematode embryos
(2017-02)The lack of physiological recordings from Caenorhabditis elegans embryos stands in stark contrast to the comprehensive anatomical and gene expression datasets already available. Using light-sheet fluorescence microscopy ... -
Stage-discharge relationship in tidal channels
(2016-12)Long-term records of the flow of water through tidal channels are essential to constrain the budgets of sediments and biogeochemical compounds in salt marshes. Statistical models which relate discharge to water level ... -
Changes in substrate availability drive carbon cycle response to chronic warming
(Elsevier, 2017-03-22)As earth's climate continues to warm, it is important to understand how the capacity of terrestrial ecosystems to retain carbon (C) will be affected. We combined measurements of microbial activity with the concentration, ... -
Luteinizing Hormone is an effective replacement for hCG to induce ovulation in Xenopus
(Elsevier, 2016-06-02)Injection of human Chorionic Gonadotropin (hCG) directly into the dorsal lymph sac of Xenopus is a commonly used protocol for induction of ovulation, but recent shortages in the stocks of commercially available hCG as well ... -
Generation of a Xenopus laevis F1 albino J strain by genome editing and oocyte host-transfer
(Elsevier, 2016-03-15)Completion of the Xenopus laevis genome sequence from inbred J strain animals has facilitated the generation of germline mutant X. laevis using targeted genome editing. In the last few years, numerous reports have demonstrated ... -
A gradient of nutrient enrichment reveals nonlinear impacts of fertilization on Arctic plant diversity and ecosystem function
(John Wiley & Sons, 2017-03-22)Rapid environmental change at high latitudes is predicted to greatly alter the diversity, structure, and function of plant communities, resulting in changes in the pools and fluxes of nutrients. In Arctic tundra, increased ... -
Diel plant water use and competitive soil cation exchange interact to enhance NH4+ and K+ availability in the rhizosphere
(Springer, 2016-11-12)Hydro-biogeochemical processes in the rhizosphere regulate nutrient and water availability, and thus ecosystem productivity. We hypothesized that two such processes often neglected in rhizosphere models — diel plant water ... -
Transcriptomes reflect the phenotypes of undifferentiated, granulocyte and macrophage forms of HL-60/S4 cells
(2017-01)In order to understand the chromatin changes underlying differential gene expression during induced differentiation of human leukemic HL-60/S4 cells, we conducted RNA-Seq analysis on quadruplicate cultures of undifferentiated, ... -
Nucleosome repositioning during differentiation of a human myeloid leukemia cell line
(Taylor & Francis, 2017-02-23)Cell differentiation is associated with changes in chromatin organization and gene expression. In this study, we examine chromatin structure following differentiation of the human myeloid leukemia cell line (HL-60/S4) ... -
Tracking microbial colonization in fecal microbiota transplantation experiments via genome-resolved metagenomics
(BioMed Central, 2017-05-04)Fecal microbiota transplantation (FMT) is an effective treatment for recurrent Clostridium difficile infection and shows promise for treating other medical conditions associated with intestinal dysbioses. However, we lack ... -
Shrub encroachment in Arctic tundra : Betula nana effects on above- and belowground litter decomposition
(John Wiley & Sons, 2017-04-07)Rapid arctic vegetation change as a result of global warming includes an increase in the cover and biomass of deciduous shrubs. Increases in shrub abundance will result in a proportional increase of shrub litter in the ... -
Seasonal variations of leaf and canopy properties tracked by ground-based NDVI imagery in a temperate forest
(Nature Publishing Group, 2017-04-28)Changes in plant phenology affect the carbon flux of terrestrial forest ecosystems due to the link between the growing season length and vegetation productivity. Digital camera imagery, which can be acquired frequently, ...
