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The Marine Biological Laboratory is an international center for research, education, and training in biology, biomedicine, and ecology.
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Visualizing calcium flux in freely moving nematode embryos
(2017-02)The lack of physiological recordings from Caenorhabditis elegans embryos stands in stark contrast to the comprehensive anatomical and gene expression datasets already available. Using light-sheet fluorescence microscopy ... -
Stage-discharge relationship in tidal channels
(2016-12)Long-term records of the flow of water through tidal channels are essential to constrain the budgets of sediments and biogeochemical compounds in salt marshes. Statistical models which relate discharge to water level ... -
Changes in substrate availability drive carbon cycle response to chronic warming
(Elsevier, 2017-03-22)As earth's climate continues to warm, it is important to understand how the capacity of terrestrial ecosystems to retain carbon (C) will be affected. We combined measurements of microbial activity with the concentration, ... -
Luteinizing Hormone is an effective replacement for hCG to induce ovulation in Xenopus
(Elsevier, 2016-06-02)Injection of human Chorionic Gonadotropin (hCG) directly into the dorsal lymph sac of Xenopus is a commonly used protocol for induction of ovulation, but recent shortages in the stocks of commercially available hCG as well ... -
Generation of a Xenopus laevis F1 albino J strain by genome editing and oocyte host-transfer
(Elsevier, 2016-03-15)Completion of the Xenopus laevis genome sequence from inbred J strain animals has facilitated the generation of germline mutant X. laevis using targeted genome editing. In the last few years, numerous reports have demonstrated ... -
A gradient of nutrient enrichment reveals nonlinear impacts of fertilization on Arctic plant diversity and ecosystem function
(John Wiley & Sons, 2017-03-22)Rapid environmental change at high latitudes is predicted to greatly alter the diversity, structure, and function of plant communities, resulting in changes in the pools and fluxes of nutrients. In Arctic tundra, increased ... -
Diel plant water use and competitive soil cation exchange interact to enhance NH4+ and K+ availability in the rhizosphere
(Springer, 2016-11-12)Hydro-biogeochemical processes in the rhizosphere regulate nutrient and water availability, and thus ecosystem productivity. We hypothesized that two such processes often neglected in rhizosphere models — diel plant water ... -
Transcriptomes reflect the phenotypes of undifferentiated, granulocyte and macrophage forms of HL-60/S4 cells
(2017-01)In order to understand the chromatin changes underlying differential gene expression during induced differentiation of human leukemic HL-60/S4 cells, we conducted RNA-Seq analysis on quadruplicate cultures of undifferentiated, ... -
Nucleosome repositioning during differentiation of a human myeloid leukemia cell line
(Taylor & Francis, 2017-02-23)Cell differentiation is associated with changes in chromatin organization and gene expression. In this study, we examine chromatin structure following differentiation of the human myeloid leukemia cell line (HL-60/S4) ... -
Tracking microbial colonization in fecal microbiota transplantation experiments via genome-resolved metagenomics
(BioMed Central, 2017-05-04)Fecal microbiota transplantation (FMT) is an effective treatment for recurrent Clostridium difficile infection and shows promise for treating other medical conditions associated with intestinal dysbioses. However, we lack ... -
Shrub encroachment in Arctic tundra : Betula nana effects on above- and belowground litter decomposition
(John Wiley & Sons, 2017-04-07)Rapid arctic vegetation change as a result of global warming includes an increase in the cover and biomass of deciduous shrubs. Increases in shrub abundance will result in a proportional increase of shrub litter in the ... -
Seasonal variations of leaf and canopy properties tracked by ground-based NDVI imagery in a temperate forest
(Nature Publishing Group, 2017-04-28)Changes in plant phenology affect the carbon flux of terrestrial forest ecosystems due to the link between the growing season length and vegetation productivity. Digital camera imagery, which can be acquired frequently, ... -
Trade-off between transcriptome plasticity and genome evolution in cephalopods
(2017-03)RNA editing, a post-transcriptional process, allows the diversification of proteomes beyond the genomic blueprint; however it is infrequently used among animals. Recent reports suggesting increased levels of RNA editing ... -
Slowed biogeochemical cycling in sub-arctic birch forest linked to reduced mycorrhizal growth and community change after a defoliation event
(Springer, 2016-08-25)Sub-arctic birch forests (Betula pubescens Ehrh. ssp. czerepanovii) periodically suffer large-scale defoliation events caused by the caterpillars of the geometrid moths Epirrita autumnata and Operophtera brumata. Despite ... -
Regulation of motor proteins, axonal transport deficits and adult-onset neurodegenerative diseases
(2017-04)Neurons affected in a wide variety of unrelated adult-onset neurodegenerative diseases (AONDs) typically exhibit a “dying back” pattern of degeneration, which is characterized by early deficits in synaptic function ... -
Achiral symmetry breaking and positive Gaussian modulus lead to scalloped colloidal membranes
(2016-11)In the presence of a non-adsorbing polymer, monodisperse rod-like particles assemble into colloidal membranes, which are one rod-length thick liquid-like monolayers of aligned rods. Unlike 3D edgeless bilayer vesicles, ... -
Modeling for understanding v. modeling for numbers
(2016-11)I draw a distinction between Modeling for Numbers, which aims to address how much, when, and where questions, and Modeling for Understanding, which aims to address how and why questions. For-numbers models are often ... -
LTR-retrotransposons from Bdelloid rotifers capture additional ORFs shared between highly diverse retroelement types
(MDPI AG, 2017-04-11)Rotifers of the class Bdelloidea, microscopic freshwater invertebrates, possess a highlydiversified repertoire of transposon families, which, however, occupy less than 4% of genomic DNA in the sequenced representative ... -
Changeable camouflage : how well can flounder resemble the colour and spatial scale of substrates in their natural habitats?
(Royal Society, 2017-03-08)Flounder change colour and pattern for camouflage. We used a spectrometer to measure reflectance spectra and a digital camera to capture body patterns of two flounder species camouflaged on four natural backgrounds of ... -
A few Pseudomonas oligotypes dominate in the meat and dairy processing environment
(Frontiers Media, 2017-03-02)The occurrence of bacteria in the food processing environments plays a key role in food contamination and development of spoilage. Species of the genus Pseudomonas are recognized as major food spoilers and the capability ...
