Burmester Thorsten

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Burmester
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Thorsten
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  • Article
    The nerve hemoglobin of the bivalve mollusc Spisula solidissima : molecular cloning, ligand binding studies, and phylogenetic analysis
    (American Society for Biochemistry and Molecular Biology, 2005-12-13) Dewilde, Sylvia ; Ebner, Bettina ; Vinck, Evi ; Gilany, Kambiz ; Hankeln, Thomas ; Burmester, Thorsten ; Kreiling, Jill A. ; Reinisch, Carol L. ; Vanfleteren, Jacques R. ; Kiger, Laurent ; Marden, Michael C. ; Hundahl, Christian ; Fago, Angela ; Van Doorslaer, Sabine ; Moens, Luc
    Members of the hemoglobin (Hb) superfamily are present in nerve tissue of several vertebrate and invertebrate species. In vertebrates they display hexacoordinate heme iron atoms and are typically expressed at low levels (µM). Their function is still a matter of debate. In invertebrates they have a hexa- or pentacoordinate heme iron, are mostly expressed at high levels (mM), and have been suggested to have a myoglobin-like function. The native Hb of the surf clam, Spisula solidissima, composed of 162 amino acids, does not show specific deviations from the globin templates. UV-visible and resonance Raman spectroscopy demonstrate a hexacoordinate heme iron. Based on the sequence analogy, the histidine E7 is proposed as a sixth ligand. Kinetic and equilibrium measurements show a moderate oxygen affinity (P50 ~0.6 torr) and no cooperativity. The histidine binding affinity is 100-fold lower than in neuroglobin. Phylogenetic analysis demonstrates a clustering of the S. solidissima nerve Hb with mollusc Hbs and myoglobins, but not with the vertebrate neuroglobins. We conclude that invertebrate nerve Hbs expressed at high levels are, despite the hexacoordinate nature of their heme iron, not essentially different from other intracellular Hbs. They most likely fulfill a myoglobin-like function and enhance oxygen supply to the neurons
  • Article
    The African coelacanth genome provides insights into tetrapod evolution
    (Nature Publishing Group, 2013-04-17) Amemiya, Chris T. ; Alfoldi, Jessica ; Lee, Alison P. ; Fan, Shaohua ; Philippe, Herve ; MacCallum, Iain ; Braasch, Ingo ; Manousaki, Tereza ; Schneider, Igor ; Rohner, Nicolas ; Organ, Chris ; Chalopin, Domitille ; Smith, Jeramiah J. ; Robinson, Mark ; Dorrington, Rosemary A. ; Gerdol, Marco ; Aken, Bronwen ; Assunta Biscotti, Maria ; Barucca, Marco ; Baurain, Denis ; Berlin, Aaron M. ; Blatch, Gregory L. ; Buonocore, Francesco ; Burmester, Thorsten ; Campbell, Michael S. ; Canapa, Adriana ; Cannon, John P. ; Christoffels, Alan ; De Moro, Gianluca ; Edkins, Adrienne L. ; Fan, Lin ; Fausto, Anna Maria ; Feiner, Nathalie ; Forconi, Mariko ; Gamieldien, Junaid ; Gnerre, Sante ; Gnirke, Andreas ; Goldstone, Jared V. ; Haerty, Wilfried ; Hahn, Mark E. ; Hesse, Uljana ; Hoffmann, Steve ; Johnson, Jeremy ; Karchner, Sibel I. ; Kuraku, Shigehiro ; Lara, Marcia ; Levin, Joshua Z. ; Litman, Gary W. ; Mauceli, Evan ; Miyake, Tsutomu ; Mueller, M. Gail ; Nelson, David R. ; Nitsche, Anne ; Olmo, Ettore ; Ota, Tatsuya ; Pallavicini, Alberto ; Panji, Sumir ; Picone, Barbara ; Ponting, Chris P. ; Prohaska, Sonja J. ; Przybylski, Dariusz ; Ratan Saha, Nil ; Ravi, Vydianathan ; Ribeiro, Filipe J. ; Sauka-Spengler, Tatjana ; Scapigliati, Giuseppe ; Searle, Stephen M. J. ; Sharpe, Ted ; Simakov, Oleg ; Stadler, Peter F. ; Stegeman, John J. ; Sumiyama, Kenta ; Tabbaa, Diana ; Tafer, Hakim ; Turner-Maier, Jason ; van Heusden, Peter ; White, Simon ; Williams, Louise ; Yandell, Mark ; Brinkmann, Henner ; Volff, Jean-Nicolas ; Tabin, Clifford J. ; Shubin, Neil ; Schartl, Manfred ; Jaffe, David B. ; Postlethwait, John H. ; Venkatesh, Byrappa ; Di Palma, Federica ; Lander, Eric S. ; Meyer, Axel ; Lindblad-Toh, Kerstin
    The discovery of a living coelacanth specimen in 1938 was remarkable, as this lineage of lobe-finned fish was thought to have become extinct 70 million years ago. The modern coelacanth looks remarkably similar to many of its ancient relatives, and its evolutionary proximity to our own fish ancestors provides a glimpse of the fish that first walked on land. Here we report the genome sequence of the African coelacanth, Latimeria chalumnae. Through a phylogenomic analysis, we conclude that the lungfish, and not the coelacanth, is the closest living relative of tetrapods. Coelacanth protein-coding genes are significantly more slowly evolving than those of tetrapods, unlike other genomic features. Analyses of changes in genes and regulatory elements during the vertebrate adaptation to land highlight genes involved in immunity, nitrogen excretion and the development of fins, tail, ear, eye, brain and olfaction. Functional assays of enhancers involved in the fin-to-limb transition and in the emergence of extra-embryonic tissues show the importance of the coelacanth genome as a blueprint for understanding tetrapod evolution.