Highly conserved molecular pathways, including Wnt signaling, promote functional recovery from spinal cord injury in lampreys
Herman, Paige E.
Bryant, Stephanie A.
Waterbury, Courtney K. M.
Herdy, Joseph R.
Arcese, Anthony A.
Buxbaum, Joseph D.
Smith, Jeramiah J.
Morgan, Jennifer R.
Bloom, Ona E.
MetadataShow full item record
In mammals, spinal cord injury (SCI) leads to dramatic losses in neurons and synaptic connections, and consequently function. Unlike mammals, lampreys are vertebrates that undergo spontaneous regeneration and achieve functional recovery after SCI. Therefore our goal was to determine the complete transcriptional responses that occur after SCI in lampreys and to identify deeply conserved pathways that promote regeneration. We performed RNA-Seq on lamprey spinal cord and brain throughout the course of functional recovery. We describe complex transcriptional responses in the injured spinal cord, and somewhat surprisingly, also in the brain. Transcriptional responses to SCI in lampreys included transcription factor networks that promote peripheral nerve regeneration in mammals such as Atf3 and Jun. Furthermore, a number of highly conserved axon guidance, extracellular matrix, and proliferation genes were also differentially expressed after SCI in lampreys. Strikingly, ~3% of differentially expressed transcripts belonged to the Wnt pathways. These included members of the Wnt and Frizzled gene families, and genes involved in downstream signaling. Pharmacological inhibition of Wnt signaling inhibited functional recovery, confirming a critical role for this pathway. These data indicate that molecular signals present in mammals are also involved in regeneration in lampreys, supporting translational relevance of the model.
© The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Scientific Reports 8 (2018): 742, doi:10.1038/s41598-017-18757-1.
Suggested CitationArticle: Herman, Paige E., Papatheodorou, Angelos, Bryant, Stephanie A., Waterbury, Courtney K. M., Herdy, Joseph R., Arcese, Anthony A., Buxbaum, Joseph D., Smith, Jeramiah J., Morgan, Jennifer R., Bloom, Ona E., "Highly conserved molecular pathways, including Wnt signaling, promote functional recovery from spinal cord injury in lampreys", Scientific Reports 8 (2018): 742, DOI:10.1038/s41598-017-18757-1, https://hdl.handle.net/1912/9505
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
GABA promotes survival and axonal regeneration in identifiable descending neurons after spinal cord injury in larval lampreys Romaus-Sanjurjo, Daniel; Ledo-García, Rocío; Fernández-López, Blanca; Hanslik, Kendra; Morgan, Jennifer R.; Barreiro-Iglesias, Antón; Rodicio, María Celina (Nature Publishing Group, 2018-06-28)The poor regenerative capacity of descending neurons is one of the main causes of the lack of recovery after spinal cord injury (SCI). Thus, it is of crucial importance to find ways to promote axonal regeneration. In ...
Hanslik, Kendra; Allen, Scott R.; Harkenrider, Tessa L.; Fogerson, Stephanie M.; Guadarrama, Eduardo; Morgan, Jennifer R. (Public Library of Science, 2019-01-30)The resilience of regeneration in vertebrates is not very well understood. Yet understanding if tissues can regenerate after repeated insults, and identifying limitations, is important for elucidating the underlying ...