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dc.contributor.authorLaFountain, James R.  Concept link
dc.contributor.authorCohan, Christopher S.  Concept link
dc.contributor.authorOldenbourg, Rudolf  Concept link
dc.identifier.citationMolecular Biology of the Cell 22 (2011): 4801-4808en_US
dc.description© The Author(s), 2011. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Molecular Biology of the Cell 22 (2011): 4801-4808, doi:10.1091/mbc.E11-06-0494.en_US
dc.description.abstractThe impact of mechanical forces on kinetochore motility was investigated using laser microsurgery to detach kinetochores with associated chromatin (K fragment) from meiotic chromosomes in spermatocytes from the crane fly Nephrotoma suturalis. In spermatocytes, elastic tethers connect telomeres of homologues during anaphase A of meiosis I, thus preventing complete disjunction until mid- to late anaphase A. K fragments liberated from tethered arms moved at twice the normal velocity toward their connected poles. To assess functional states of detached and control kinetochores, we loaded cells with fluorescently labeled tubulin for fluorescent speckle microscopy on kinetochore microtubules. Control kinetochores added fluorescent speckles at the kinetochore during anaphase A, whereas kinetochores of K fragments generally did not. In cases in which speckles reappeared in K-fragment K fibers, speckles and K fragments moved poleward at similar velocities. Thus detached kinetochores convert from their normal polymerization (reverse pac-man) state to a different state, in which polymerization is not evident. We suggest that the converted state is “park,” in which kinetochores are anchored to plus ends of kinetochore microtubules that shorten exclusively at their polar ends.en_US
dc.description.sponsorshipThis study was supported by a grant to R.O. from the National Institute of Biomedical Imaging and Bioengineering (R01EB002583-17).en_US
dc.publisherAmerican Society of Cell Biologyen_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.titleFunctional states of kinetochores revealed by laser microsurgery and fluorescent speckle microscopyen_US

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