Microfluidic encapsulation of Xenopus laevis cell-free extracts using hydrogel photolithography
Microfluidic encapsulation of Xenopus laevis cell-free extracts using hydrogel photolithography
Date
2020-12-18
Authors
Geisterfer, Zachary M.
Oakey, John
Gatlin, Jesse C.
Oakey, John
Gatlin, Jesse C.
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DOI
10.1016/j.xpro.2020.100221
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Keywords
Biophysics
Cell Biology
Cell isolation
Microscopy
Model Organisms
Cell Biology
Cell isolation
Microscopy
Model Organisms
Abstract
Cell-free extract derived from the eggs of the African clawed frog Xenopus laevis is a well-established model system that has been used historically in bulk aliquots. Here, we describe a microfluidic approach for isolating discrete, biologically relevant volumes of cell-free extract, with more expansive and precise control of extract shape compared with extract-oil emulsions. This approach is useful for investigating the mechanics of intracellular processes affected by cell geometry or cytoplasmic volume, including organelle scaling and positioning mechanisms.
For complete details on the use and execution of this protocol, please refer to Geisterfer et al. (2020).
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© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Geisterfer, Z. M., Oakey, J., & Gatlin, J. C. . Microfluidic encapsulation of Xenopus laevis cell-free extracts using hydrogel photolithography. STAR Protocols, 1(3), (2020): 100221, doi:10.1016/j.xpro.2020.100221.
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Geisterfer, Z. M., Oakey, J., & Gatlin, J. C. (2020). Microfluidic encapsulation of Xenopus laevis cell-free extracts using hydrogel photolithography. STAR Protocols, 1(3), 100221.
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