Evidence for substantial intramolecular heterogeneity in the stable carbon isotopic composition of phytol in photoautotrophic organisms
van der Meer, Marcel T. J.
Hayes, John M.
Sinninghe Damste, Jaap S.
MetadataShow full item record
KeywordPhytol; Phytane; Pristane; Stable carbon isotope; Mevalonic acid pathway; 2-C-methylerythritol-4-phosphate pathway; 2,6,10-trimethylpentadecan-2-one
The ubiquitous isoprenoid phytol was isolated from a range of algae, terrestrial plants and a bacterium and its two terminal carbon atoms were quantitatively removed by chemical oxidation. The product, 6,10,14-trimethylpentadecan-2-one, was depleted in 13C by 1-4‰ relative to the parent phytol. This difference is significant, and indicates that the pathway for biosynthesis of phytol induces substantial intramolecular stable carbon isotopic fractionations. The nature and magnitude of the fractionations suggest strongly that it is associated both with the biosynthesis of isopentenyl pyrophosphate via the 2-C-methylerythritol-4-phosphate pathway and with the formation of carotenoids and phytol from geranyl-geraniolphosphate. As a result of these large, intramolecular isotopic differences, diagenetic products formed by loss of C, such as pristane, may be naturally depleted in 13C by several permil relative to phytane.
Author Posting. © Elsevier B.V., 2007. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Organic Geochemistry 39 (2008): 135-146, doi:10.1016/j.orggeochem.2007.09.002.
Showing items related by title, author, creator and subject.
Variability in the carbon isotopic composition of foliage carbon pools (soluble carbohydrates, waxes) and respiration fluxes in southeastern U.S. pine forests Mortazavi, Behzad; Conte, Maureen H.; Chanton, Jeffrey P.; Weber, John C.; Martin, Timothy A.; Cropper, Wendell P. (American Geophysical Union, 2012-04-19)We measured the δ13C of assimilated carbon (foliage organic matter (δCOM), soluble carbohydrates (δCSC), and waxes (δCW)) and respiratory carbon (foliage (δCFR), soil (δCSR) and ecosystem 13CO2 (δCER)) for two years at ...
Carbon and nitrogen isotope fractionation of amino acids in an avian marine predator, the gentoo penguin (Pygoscelis papua) McMahon, Kelton W.; Polito, Michael J.; Abel, Stephanie; McCarthy, Matthew D.; Thorrold, Simon R. (John Wiley & Sons, 2015-02-25)Compound-specific stable isotope analysis (CSIA) of amino acids (AA) has rapidly become a powerful tool in studies of food web architecture, resource use, and biogeochemical cycling. However, applications to avian ecology ...
The role of biological production in pleistocene atmospheric carbon dioxide variations and the nitrogen isotope dynamics of the southern ocean Sigman, Daniel M. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1997-09)This dissertation contributes to the search for a cause of glacial/interglacial variations in atmospheric carbon dioxide. The hypotheses addressed involve changes in low and high-latitude biological export production. A ...