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    Roles of polymerization dynamics, opposed motors, and a tensile element in governing the length of Xenopus extract meiotic spindles

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    Date
    2005-03-23
    Author
    Mitchison, Timothy J.  Concept link
    Maddox, P.  Concept link
    Gaetz, J.  Concept link
    Groen, Aaron C.  Concept link
    Shirasu, M.  Concept link
    Desai, Ankur R.  Concept link
    Salmon, Edward D.  Concept link
    Kapoor, Tarun M.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/1934
    As published
    https://doi.org/10.1091/mbc.E05-02-0174
    DOI
    10.1091/mbc.E05-02-0174
    Abstract
    Metaphase spindles assemble to a steady state in length by mechanisms that involve microtubule dynamics and motor proteins, but they are incompletely understood. We found that Xenopus extract spindles recapitulate the length of egg meiosis II spindles, by using mechanisms intrinsic to the spindle. To probe these mechanisms, we perturbed microtubule polymerization dynamics and opposed motor proteins and measured effects on spindle morphology and dynamics. Microtubules were stabilized by hexylene glycol and inhibition of the catastrophe factor mitotic centromere-associated kinesin (MCAK) (a kinesin 13, previously called XKCM) and destabilized by depolymerizing drugs. The opposed motors Eg5 and dynein were inhibited separately and together. Our results are consistent with important roles for polymerization dynamics in regulating spindle length, and for opposed motors in regulating the relative stability of bipolar versus monopolar organization. The response to microtubule destabilization suggests that an unidentified tensile element acts in parallel with these conventional factors, generating spindle shortening force.
    Description
    Author Posting. © American Society for Cell Biology, 2005. This article is posted here by permission of American Society for Cell Biology for personal use, not for redistribution. The definitive version was published in Molecular Biology of the Cell 16 (2005): 3064-3076, doi:10.1091/mbc.E05-02-0174.
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    • Cellular Dynamics Program
    Suggested Citation
    Molecular Biology of the Cell 16 (2005): 3064-3076
     
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