Shared up-regulation and contrasting down-regulation of gene expression distinguish desiccation-tolerant from intolerant green algae

dc.contributor.author Peredo, Elena L.
dc.contributor.author Cardon, Zoe G.
dc.date.accessioned 2020-07-29T20:50:44Z
dc.date.available 2020-07-29T20:50:44Z
dc.date.issued 2020-07-21
dc.description © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Peredo, E. L., & Cardon, Z. G. Shared up-regulation and contrasting down-regulation of gene expression distinguish desiccation-tolerant from intolerant green algae. Proceedings of the National Academy of Sciences of the United States of America, 117(29), 1(2020): 7438-17445, doi:10.1073/pnas.1906904117. en_US
dc.description.abstract Among green plants, desiccation tolerance is common in seeds and spores but rare in leaves and other vegetative green tissues. Over the last two decades, genes have been identified whose expression is induced by desiccation in diverse, desiccation-tolerant (DT) taxa, including, e.g., late embryogenesis abundant proteins (LEA) and reactive oxygen species scavengers. This up-regulation is observed in DT resurrection plants, mosses, and green algae most closely related to these Embryophytes. Here we test whether this same suite of protective genes is up-regulated during desiccation in even more distantly related DT green algae, and, importantly, whether that up-regulation is unique to DT algae or also occurs in a desiccation-intolerant relative. We used three closely related aquatic and desert-derived green microalgae in the family Scenedesmaceae and capitalized on extraordinary desiccation tolerance in two of the species, contrasting with desiccation intolerance in the third. We found that during desiccation, all three species increased expression of common protective genes. The feature distinguishing gene expression in DT algae, however, was extensive down-regulation of gene expression associated with diverse metabolic processes during the desiccation time course, suggesting a switch from active growth to energy-saving metabolism. This widespread downshift did not occur in the desiccation-intolerant taxon. These results show that desiccation-induced up-regulation of expression of protective genes may be necessary but is not sufficient to confer desiccation tolerance. The data also suggest that desiccation tolerance may require induced protective mechanisms operating in concert with massive down-regulation of gene expression controlling numerous other aspects of metabolism. en_US
dc.description.sponsorship Dr. Louise Lewis (University of Connecticut) provided F. rotunda and A. deserticola. Suzanne Thomas and Jordan Stark provided expert technical assistance. This work was supported by the NSF, Division of Integrative Organismal Systems (1355085 to Z.G.C.), and an anonymous donor (to Z.G.C.). en_US
dc.identifier.citation Peredo, E. L., & Cardon, Z. G. (2020). Shared up-regulation and contrasting down-regulation of gene expression distinguish desiccation-tolerant from intolerant green algae. Proceedings of the National Academy of Sciences of the United States of America, 117(29), 17438-17445. en_US
dc.identifier.doi 10.1073/pnas.1906904117
dc.identifier.uri https://hdl.handle.net/1912/26026
dc.publisher National Academy of Sciences en_US
dc.relation.uri https://doi.org/10.1073/pnas.1906904117
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject Aquatic green algae en_US
dc.subject Desert-evolved green algae en_US
dc.subject Extremophiles en_US
dc.subject Microbiotic en_US
dc.subject Crusts en_US
dc.subject Scenedesmaceae en_US
dc.title Shared up-regulation and contrasting down-regulation of gene expression distinguish desiccation-tolerant from intolerant green algae en_US
dc.type Article en_US
dspace.entity.type Publication
relation.isAuthorOfPublication 46299fbe-4cda-4abb-8adf-f290218bf683
relation.isAuthorOfPublication 77581573-d1d0-4c71-8421-c4f03d4afff5
relation.isAuthorOfPublication.latestForDiscovery 46299fbe-4cda-4abb-8adf-f290218bf683
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