Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses

dc.contributor.author Kim, Jinsook
dc.contributor.author Lee, Soojung
dc.contributor.author Tsuda, Sachiko
dc.contributor.author Zhang, Xuying
dc.contributor.author Asrican, Brent
dc.contributor.author Gloss, Bernd
dc.contributor.author Feng, Guoping
dc.contributor.author Augustine, George J.
dc.date.accessioned 2014-06-20T19:21:20Z
dc.date.available 2014-06-20T19:21:20Z
dc.date.issued 2014-05-22
dc.description © The Author(s), 2014. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Cell 7 (2014): 1601–1613, doi:10.1016/j.celrep.2014.04.047. en_US
dc.description.abstract We used high-speed optogenetic mapping technology to examine the spatial organization of local inhibitory circuits formed by cerebellar interneurons. Transgenic mice expressing channelrhodopsin-2 exclusively in molecular layer interneurons allowed us to focally photostimulate these neurons, while measuring resulting responses in postsynaptic Purkinje cells. This approach revealed that interneurons converge upon Purkinje cells over a broad area and that at least seven interneurons form functional synapses with a single Purkinje cell. The number of converging interneurons was reduced by treatment with gap junction blockers, revealing that electrical synapses between interneurons contribute substantially to the spatial convergence. Remarkably, gap junction blockers affected convergence in sagittal slices, but not in coronal slices, indicating a sagittal bias in electrical coupling between interneurons. We conclude that electrical synapse networks spatially coordinate interneurons in the cerebellum and may also serve this function in other brain regions. en_US
dc.description.sponsorship This work was supported by a CRP grant from the National Research Foundation of Singapore and by the World Class Institute (WCI) Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology of Korea (NRF grant number WCI 2009-003). en_US
dc.format.mimetype application/pdf
dc.identifier.citation Cell 7 (2014): 1601–1613 en_US
dc.identifier.doi 10.1016/j.celrep.2014.04.047
dc.identifier.uri https://hdl.handle.net/1912/6707
dc.language.iso en_US en_US
dc.publisher Cell Press en_US
dc.relation.uri https://doi.org/10.1016/j.celrep.2014.04.047
dc.rights Attribution 3.0 Unported
dc.rights.uri http://creativecommons.org/licenses/by/3.0/
dc.title Optogenetic mapping of cerebellar inhibitory circuitry reveals spatially biased coordination of interneurons via electrical synapses en_US
dc.type Article en_US
dspace.entity.type Publication
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