Anvi’o : an advanced analysis and visualization platform for ‘omics data

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Date
2015-10-08
Authors
Eren, A. Murat
Esen, Ozcan C.
Quince, Christopher
Vineis, Joseph H.
Morrison, Hilary G.
Sogin, Mitchell L.
Delmont, Tom O.
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DOI
10.7717/peerj.1319
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Keywords
Metagenomics
Assembly
Genome binning
Visualization
SNP profiling
Metatranscriptomics
Abstract
Advances in high-throughput sequencing and ‘omics technologies are revolutionizing studies of naturally occurring microbial communities. Comprehensive investigations of microbial lifestyles require the ability to interactively organize and visualize genetic information and to incorporate subtle differences that enable greater resolution of complex data. Here we introduce anvi’o, an advanced analysis and visualization platform that offers automated and human-guided characterization of microbial genomes in metagenomic assemblies, with interactive interfaces that can link ‘omics data from multiple sources into a single, intuitive display. Its extensible visualization approach distills multiple dimensions of information about each contig, offering a dynamic and unified work environment for data exploration, manipulation, and reporting. Using anvi’o, we re-analyzed publicly available datasets and explored temporal genomic changes within naturally occurring microbial populations through de novo characterization of single nucleotide variations, and linked cultivar and single-cell genomes with metagenomic and metatranscriptomic data. Anvi’o is an open-source platform that empowers researchers without extensive bioinformatics skills to perform and communicate in-depth analyses on large ‘omics datasets.
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© The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PeerJ 3 (2015): e1319, doi:10.7717/peerj.1319.
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PeerJ 3 (2015): e1319
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Except where otherwise noted, this item's license is described as Attribution 4.0 International