Transcriptome analyses of the Giardia lamblia life cycle
Transcriptome analyses of the Giardia lamblia life cycle
Date
2010-06-04
Authors
Birkeland, Shanda R.
Preheim, Sarah P.
Davids, Barbara J.
Cipriano, Michael J.
Palm, Daniel
Reiner, David S.
Svard, Staffan G.
Gillin, Frances D.
McArthur, Andrew G.
Preheim, Sarah P.
Davids, Barbara J.
Cipriano, Michael J.
Palm, Daniel
Reiner, David S.
Svard, Staffan G.
Gillin, Frances D.
McArthur, Andrew G.
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Keywords
Transcriptome
Gene expression
Life cycle
SAGE
Gene expression
Life cycle
SAGE
Abstract
We quantified mRNA abundance from 10 stages in the Giardia lamblia life cycle in vitro using
Serial Analysis of Gene Expression (SAGE). 163 abundant transcripts were expressed
constitutively. 71 transcripts were upregulated specifically during excystation and 42 during
encystation. Nonetheless, the transcriptomes of cysts and trophozoites showed major
differences. SAGE detected co-expressed clusters of 284 transcripts differentially expressed in
cysts and excyzoites and 287 transcripts in vegetative trophozoites and encysting cells. All
clusters included known genes and pathways as well as proteins unique to Giardia or
diplomonads. SAGE analysis of the Giardia life cycle identified a number of kinases,
phosphatases, and DNA replication proteins involved in excystation and encystation, which
could be important for examining the roles of cell signaling in giardial differentiation. Overall,
these data pave the way for directed gene discovery and a better understanding of the biology
of Giardia lamblia.
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Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Molecular and Biochemical Parasitology 174 (2010): 62-65, doi:10.1016/j.molbiopara.2010.05.010.