Transcriptome analyses of the Giardia lamblia life cycle

dc.contributor.author Birkeland, Shanda R.
dc.contributor.author Preheim, Sarah P.
dc.contributor.author Davids, Barbara J.
dc.contributor.author Cipriano, Michael J.
dc.contributor.author Palm, Daniel
dc.contributor.author Reiner, David S.
dc.contributor.author Svard, Staffan G.
dc.contributor.author Gillin, Frances D.
dc.contributor.author McArthur, Andrew G.
dc.date.accessioned 2010-07-14T16:03:31Z
dc.date.available 2010-07-14T16:03:31Z
dc.date.issued 2010-06-04
dc.description Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Molecular and Biochemical Parasitology 174 (2010): 62-65, doi:10.1016/j.molbiopara.2010.05.010. en_US
dc.description.abstract We quantified mRNA abundance from 10 stages in the Giardia lamblia life cycle in vitro using Serial Analysis of Gene Expression (SAGE). 163 abundant transcripts were expressed constitutively. 71 transcripts were upregulated specifically during excystation and 42 during encystation. Nonetheless, the transcriptomes of cysts and trophozoites showed major differences. SAGE detected co-expressed clusters of 284 transcripts differentially expressed in cysts and excyzoites and 287 transcripts in vegetative trophozoites and encysting cells. All clusters included known genes and pathways as well as proteins unique to Giardia or diplomonads. SAGE analysis of the Giardia life cycle identified a number of kinases, phosphatases, and DNA replication proteins involved in excystation and encystation, which could be important for examining the roles of cell signaling in giardial differentiation. Overall, these data pave the way for directed gene discovery and a better understanding of the biology of Giardia lamblia. en_US
dc.description.sponsorship BJD, DSR, and FDG were supported by NIH grants AI42488, GM61896, DK35108, and AI051687. DP and SGS were supported by grants from the Swedish Natural Science Research Council, the Swedish Medical Research Council, and the Karolinska Institutet. AGM, SRB, SPP, and MJC were supported by NIH grant AI51089 and by the Marine Biological Laboratory’s Program in Global Infectious Diseases, funded by the Ellison Medical Foundation. en_US
dc.format.mimetype application/pdf
dc.format.mimetype application/vnd.ms-excel
dc.format.mimetype image/tiff
dc.identifier.uri https://hdl.handle.net/1912/3753
dc.language.iso en_US en_US
dc.relation.uri https://doi.org/10.1016/j.molbiopara.2010.05.010
dc.subject Transcriptome en_US
dc.subject Gene expression en_US
dc.subject Life cycle en_US
dc.subject SAGE en_US
dc.title Transcriptome analyses of the Giardia lamblia life cycle en_US
dc.type Preprint en_US
dspace.entity.type Publication
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