Differential cellular responses associated with oxidative stress and cell fate decision under nitrate and phosphate limitations in Thalassiosira pseudonana : comparative proteomics

dc.contributor.author Lin, Qun
dc.contributor.author Liang, Jun-Rong
dc.contributor.author Huang, Qian-Qian
dc.contributor.author Luo, Chun-Shan
dc.contributor.author Anderson, Donald M.
dc.contributor.author Bowler, Chris
dc.contributor.author Chen, Chang-Ping
dc.contributor.author Li, Xue-Song
dc.contributor.author Gao, Ya-Hui
dc.date.accessioned 2017-10-11T16:18:56Z
dc.date.available 2017-10-11T16:18:56Z
dc.date.issued 2017-09-14
dc.description © The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS ONE 12 (2017): e0184849, doi:10.1371/journal.pone.0184849. en_US
dc.description.abstract Diatoms are important components of marine ecosystems and contribute greatly to the world's primary production. Despite their important roles in ecosystems, the molecular basis of how diatoms cope with oxidative stress caused by nutrient fluctuations remains largely unknown. Here, an isobaric tags for relative and absolute quantitation (iTRAQ) proteomic method was coupled with a series of physiological and biochemical techniques to explore oxidative stress- and cell fate decision-related cellular and metabolic responses of the diatom Thalassiosira pseudonana to nitrate (N) and inorganic phosphate (P) stresses. A total of 1151 proteins were detected; 122 and 56 were significantly differentially expressed from control under N- and P-limited conditions, respectively. In N-limited cells, responsive proteins were related to reactive oxygen species (ROS) accumulation, oxidative stress responses and cell death, corresponding to a significant decrease in photosynthetic efficiency, marked intracellular ROS accumulation, and caspase-mediated programmed cell death activation. None of these responses were identified in P-limited cells; however, a significant up-regulation of alkaline phosphatase proteins was observed, which could be the major contributor for P-limited cells to cope with ambient P deficiency. These findings demonstrate that fundamentally different metabolic responses and cellular regulations are employed by the diatom in response to different nutrient stresses and to keep the cells viable. en_US
dc.description.sponsorship This study was funded by the National Natural Science Foundation of China (41576138, 41076080, 41576138) to Dr. Jun-Rong Liang; the Woods Hole Center for Oceans and Human Health, National Science Foundation (OCE-1314642) to Dr. DonaldM Anderson; the National Institute of Environmental Health Sciences (1-P01-ES021923- 01) to Dr. DonaldM Anderson; and the ERC Advanced Award Diatomite and ANR project DiaDomOil to Dr. Chris Bowler. en_US
dc.identifier.citation PLoS ONE 12 (2017): e0184849 en_US
dc.identifier.doi 10.1371/journal.pone.0184849
dc.identifier.uri https://hdl.handle.net/1912/9294
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.relation.uri https://doi.org/10.1371/journal.pone.0184849
dc.rights Attribution 4.0 International *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ *
dc.title Differential cellular responses associated with oxidative stress and cell fate decision under nitrate and phosphate limitations in Thalassiosira pseudonana : comparative proteomics en_US
dc.type Article en_US
dspace.entity.type Publication
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