Differential cellular responses associated with oxidative stress and cell fate decision under nitrate and phosphate limitations in Thalassiosira pseudonana : comparative proteomics
Differential cellular responses associated with oxidative stress and cell fate decision under nitrate and phosphate limitations in Thalassiosira pseudonana : comparative proteomics
Date
2017-09-14
Authors
Lin, Qun
Liang, Jun-Rong
Huang, Qian-Qian
Luo, Chun-Shan
Anderson, Donald M.
Bowler, Chris
Chen, Chang-Ping
Li, Xue-Song
Gao, Ya-Hui
Liang, Jun-Rong
Huang, Qian-Qian
Luo, Chun-Shan
Anderson, Donald M.
Bowler, Chris
Chen, Chang-Ping
Li, Xue-Song
Gao, Ya-Hui
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10.1371/journal.pone.0184849
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Abstract
Diatoms are important components of marine ecosystems and contribute greatly to the
world's primary production. Despite their important roles in ecosystems, the molecular basis
of how diatoms cope with oxidative stress caused by nutrient fluctuations remains largely
unknown. Here, an isobaric tags for relative and absolute quantitation (iTRAQ) proteomic
method was coupled with a series of physiological and biochemical techniques to explore
oxidative stress- and cell fate decision-related cellular and metabolic responses of the diatom
Thalassiosira pseudonana to nitrate (N) and inorganic phosphate (P) stresses. A total
of 1151 proteins were detected; 122 and 56 were significantly differentially expressed
from control under N- and P-limited conditions, respectively. In N-limited cells, responsive
proteins were related to reactive oxygen species (ROS) accumulation, oxidative stress
responses and cell death, corresponding to a significant decrease in photosynthetic efficiency,
marked intracellular ROS accumulation, and caspase-mediated programmed cell
death activation. None of these responses were identified in P-limited cells; however, a significant
up-regulation of alkaline phosphatase proteins was observed, which could be the
major contributor for P-limited cells to cope with ambient P deficiency. These findings demonstrate
that fundamentally different metabolic responses and cellular regulations are
employed by the diatom in response to different nutrient stresses and to keep the cells
viable.
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© The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS ONE 12 (2017): e0184849, doi:10.1371/journal.pone.0184849.
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PLoS ONE 12 (2017): e0184849