Flexible and dynamic nucleosome fiber in living mammalian cells
Flexible and dynamic nucleosome fiber in living mammalian cells
dc.contributor.author | Nozaki, Tadasu | |
dc.contributor.author | Kaizu, Kazunari | |
dc.contributor.author | Pack, Chan-Gi | |
dc.contributor.author | Tamura, Sachiko | |
dc.contributor.author | Tani, Tomomi | |
dc.contributor.author | Hihara, Saera | |
dc.contributor.author | Nagai, Takeharu | |
dc.contributor.author | Takahashi, Koichi | |
dc.contributor.author | Maeshima, Kazuhiro | |
dc.date.accessioned | 2014-03-11T14:45:26Z | |
dc.date.available | 2014-03-11T14:45:26Z | |
dc.date.issued | 2013-08-12 | |
dc.description | © Landes Bioscience, 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Nucleus 4 (2013): 349-356, doi:10.4161/nucl.26053. | en_US |
dc.description.abstract | Genomic DNA is organized three dimensionally within cells as chromatin and is searched and read by various proteins by an unknown mechanism; this mediates diverse cell functions. Recently, several pieces of evidence, including our cryomicroscopy and synchrotron X-ray scattering analyses, have demonstrated that chromatin consists of irregularly folded nucleosome fibers without a 30-nm chromatin fiber (i.e., a polymer melt-like structure). This melt-like structure implies a less physically constrained and locally more dynamic state, which may be crucial for protein factors to scan genomic DNA. Using a combined approach of fluorescence correlation spectroscopy, Monte Carlo computer simulations, and single nucleosome imaging, we demonstrated the flexible and dynamic nature of the nucleosome fiber in living mammalian cells. We observed local nucleosome fluctuation (~50 nm movement per 30 ms) caused by Brownian motion. Our in vivo-in silico results suggest that local nucleosome dynamics facilitate chromatin accessibility and play a critical role in the scanning of genome information. | en_US |
dc.description.sponsorship | This work was supported by a grant-in-aid for a MEXT grant, JST CREST, Yamada Science Foundation and Takeda Science Foundation. Nozaki T and Hihara A are JSPS fellows. | en_US |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | video/mp4 | |
dc.identifier.citation | Nucleus 4 (2013): 349-356 | en_US |
dc.identifier.doi | 10.4161/nucl.26053 | |
dc.identifier.uri | https://hdl.handle.net/1912/6471 | |
dc.language.iso | en | en_US |
dc.publisher | Landes Bioscience | en_US |
dc.relation.uri | https://doi.org/10.4161/nucl.26053 | |
dc.rights | Attribution-NonCommercial 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/ | * |
dc.title | Flexible and dynamic nucleosome fiber in living mammalian cells | en_US |
dc.type | Article | en_US |
dspace.entity.type | Publication | |
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