Biochemical characterisation of sis: a distinct thermophilic PETase with enhanced nanoPET substrate hydrolysis and thermal stability
Biochemical characterisation of sis: a distinct thermophilic PETase with enhanced nanoPET substrate hydrolysis and thermal stability
Date
2024-07-25
Authors
Ercolano, Carmen
Iacono, Roberta
Cafaro, Valeria
Pizzo, Elio
Giovannelli, Donato
Feuerriegel, Golo
Streit, Wolfgang R.
Strazzulli, Andrea
Moracci, Marco
Iacono, Roberta
Cafaro, Valeria
Pizzo, Elio
Giovannelli, Donato
Feuerriegel, Golo
Streit, Wolfgang R.
Strazzulli, Andrea
Moracci, Marco
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DOI
10.3390/ijms25158120
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PET hydrolysis
Enzyme discovery
Thermozymes
Enzyme discovery
Thermozymes
Abstract
Polyethylene terephthalate (PET) degradation by enzymatic hydrolysis is significant for addressing plastic pollution and fostering sustainable waste management practices. Identifying thermophilic and thermostable PET hydrolases is particularly crucial for industrial bioprocesses, where elevated temperatures may enhance enzymatic efficiency and process kinetics. In this study, we present the discovery of a novel thermophilic and thermostable PETase enzyme named Sis, obtained through metagenomic sequence-based analysis. Sis exhibits robust activity on nanoPET substrates, demonstrating effectiveness at temperatures up to 70 °C and displaying exceptional thermal stability with a melting temperature (Tm) of 82 °C. Phylogenetically distinct from previously characterised PET hydrolases, Sis represents a valuable addition to the repertoire of enzymes suitable for PET degradation.
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© The Author(s), 2024. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Ercolano, C., Iacono, R., Cafaro, V., Pizzo, E., Giovannelli, D., Feuerriegel, G., Streit, W., Strazzulli, A., & Moracci, M. (2024). Biochemical characterisation of sis: a distinct thermophilic PETase with enhanced nanoPET substrate hydrolysis and thermal stability. International Journal of Molecular Sciences, 25(15), 8120, https://doi.org/10.3390/ijms25158120.
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Ercolano, C., Iacono, R., Cafaro, V., Pizzo, E., Giovannelli, D., Feuerriegel, G., Streit, W., Strazzulli, A., & Moracci, M. (2024). Biochemical characterisation of sis: a distinct thermophilic PETase with enhanced nanoPET substrate hydrolysis and thermal stability. International Journal of Molecular Sciences, 25(15), 8120.
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