HPLC and fluorometric derived phytoplankton pigment concentrations from seawater collected at the Bermuda Atlantic Time-series Study (BATS) site from October 1988 through June 2024

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Date
2025-03-18
Authors
Bates, Nicholas
Johnson, Rodney J.
Lethaby, Paul J.
Medley, Claire
Smith, Dominic
Stuart, Emma
May, Rebecca
Derbyshire, Lucinda
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Date Created
2025-01-15
Location
Northwest Sargasso Sea at 31 deg 40' N, 64 deg 10' W
westlimit: -65.343; southlimit: 27.823; eastlimit: -62.718; northlimit: 33.142
DOI
10.26008/1912/bco-dmo.893521.7
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Keywords
BATS
HPLC pigments
phytoplankton pigments
algal pigments
Abstract
Data presented here contain HPLC derived phytoplankton pigments and fluorometric chlorophyll-a from the BATS site for October 1988 to June 2024. Samples are typically collected from 12 depths in the upper 250 meters of the water column where by 4l of seawater is filtered under low vacuum through a 25mm GF/F filter. The filter is then flash frozen in liquid nitrogen and stored at -80C. Shoreside, analysis is performed on a HPLC using a modified version of Wright et al., 1991 (modified by Dr R. Bidigare,University of Hawaii) which identifies the following pigments: Chlorophyll C3, Chlorophyll C2, Peridinin, 19’Butanoyloxyfucoxanthin, Fucoxanthin, 19’Hexanoyloxyfucoxanthin, Prasinoxanthin, Violoxanthin, Diadinoxanthin,, Alloxanthin, Diatoxanthin, Lutein, Zeaxanthin, Chlorophyll b, Chlorophyll a, Divinyl, chlorophyll_a, Alpha and beta carotene. Additionally, chlorophyll_A and phaeopigments are analyzed using a fluorometeric assay. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/893521
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Dataset: BATS HPLC pigments
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