Intranuclear birefringent inclusions in paraffin sections by polychromatic polarization microscopy

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2021-03-18
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Vitkunaite, Aiste
 Laurinaviciene, Aida
 Plancoulaine, Benoit
 Rasmusson, Allan
 Levenson, Richard
 Shribak, Michael
 Laurinavicius, Arvydas
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s41598-021-85667-8.pdf (4.76 MB)
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https://hdl.handle.net/1912/27584
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https://doi.org/10.1038/s41598-021-85667-8
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10.1038/s41598-021-85667-8
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Abstract
Intranuclear birefringent inclusions (IBI) found in various cell types in paraffin-embedded tissue sections have long been considered to be a tissue processing artifact, although an association with biological processes has been suggested. We applied polychromatic polarization microscopy to image their spatial organization. Our study provides evidence that IBI are caused by liquid paraffin-macromolecular crystals formed during paraffin-embedding procedures within cells and potentially reflect an active transcriptional status.
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© The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Vitkunaite, A., Laurinaviciene, A., Plancoulaine, B., Rasmusson, A., Levenson, R., Shribak, M., & Laurinavicius, A. Intranuclear birefringent inclusions in paraffin sections by polychromatic polarization microscopy. Scientific Reports, 11(1), (2021): 6275, https://doi.org/10.1038/s41598-021-85667-8.
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Vitkunaite, A., Laurinaviciene, A., Plancoulaine, B., Rasmusson, A., Levenson, R., Shribak, M., & Laurinavicius, A. (2021). Intranuclear birefringent inclusions in paraffin sections by polychromatic polarization microscopy. Scientific Reports, 11(1), 6275.
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Bell Center Publications
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