Phosphorus supply drives rapid turnover of membrane phospholipids in the diatom Thalassiosira pseudonana

Abstract

In low phosphorus (P) marine systems phytoplankton replace membrane phospholipids with non-phosphorus lipids, but it is not known how rapidly this substitution occurs. Here, when cells of the model diatom Thalassiosira pseudonana were transferred from P-replete medium to P-free medium, the phospholipid content of the cells rapidly declined within 48 h from 45±0.9% to 21±4.5% of total membrane lipids; the difference was made up by non-phosphorus lipids. Conversely, when P-limited T. pseudonana were resupplied with P, cells reduced the percentage of their total membrane lipid contributed by a non-phosphorus lipid from 43±1.5% to 7.3±0.9% within 24 h, while the contribution by phospholipids rose from 2.2±0.1% to 44±3%. This dynamic phospholipid reservoir contained sufficient P to synthesize multiple haploid genomes, suggesting that phospholipid turnover could be an important P source for cells. Field observations of phytoplankton lipid content may thus reflect short-term changes in P supply and cellular physiology, rather than simply long-term adjustment to the environment.