Diversity and spatial distribution of hydrazine oxidoreductase (hzo) gene in the oxygen minimum zone off Costa Rica
Diversity and spatial distribution of hydrazine oxidoreductase (hzo) gene in the oxygen minimum zone off Costa Rica
dc.contributor.author | Kong, Liangliang | |
dc.contributor.author | Jing, Hongmei | |
dc.contributor.author | Kataoka, Takafumi | |
dc.contributor.author | Buchwald, Carolyn | |
dc.contributor.author | Liu, Hongbin | |
dc.date.accessioned | 2013-12-05T16:53:57Z | |
dc.date.available | 2013-12-05T16:53:57Z | |
dc.date.issued | 2013-10-31 | |
dc.description | © The Author(s), 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS ONE 8 (2013): e78275, doi:10.1371/journal.pone.0078275. | en_US |
dc.description.abstract | Anaerobic ammonia oxidation (anammox) as an important nitrogen loss pathway has been reported in marine oxygen minimum zones (OMZs), but the community composition and spatial distribution of anammox bacteria in the eastern tropical North Pacific (ETNP) OMZ are poorly determined. In this study, anammox bacterial communities in the OMZ off Costa Rica (CRD-OMZ) were analyzed based on both hydrazine oxidoreductase (hzo) genes and their transcripts assigned to cluster 1 and 2. The anammox communities revealed by hzo genes and proteins in CRD-OMZ showed a low diversity. Gene quantification results showed that hzo gene abundances peaked in the upper OMZs, associated with the peaks of nitrite concentration. Nitrite and oxygen concentrations may therefore colimit the distribution of anammox bacteria in this area. Furthermore, transcriptional activity of anammox bacteria was confirmed by obtaining abundant hzo mRNA transcripts through qRT-PCR. A novel hzo cluster 2x clade was identified by the phylogenetic analysis and these novel sequences were abundant and widely distributed in this environment. Our study demonstrated that both cluster 1 and 2 anammox bacteria play an active role in the CRD-OMZ, and the cluster 1 abundance and transcriptional activity were higher than cluster 2 in both free-living and particle-attached fractions at both gene and transcriptional levels. | en_US |
dc.description.sponsorship | National Science Foundation (OCE #0961098) Hong Kong Research Grants Council (661911 and 661912) Chinese Academy of Science (SIDSSE-BR-201301). | en_US |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | application/msword | |
dc.identifier.citation | PLoS ONE 8 (2013): e78275 | en_US |
dc.identifier.doi | 10.1371/journal.pone.0078275 | |
dc.identifier.uri | https://hdl.handle.net/1912/6344 | |
dc.language.iso | en_US | en_US |
dc.publisher | Public Library of Science | en_US |
dc.relation.uri | https://doi.org/10.1371/journal.pone.0078275 | |
dc.rights | Attribution 3.0 Unported | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/ | * |
dc.title | Diversity and spatial distribution of hydrazine oxidoreductase (hzo) gene in the oxygen minimum zone off Costa Rica | en_US |
dc.type | Article | en_US |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 56cc70a6-13cb-4d22-9765-2b3a2b2f1536 | |
relation.isAuthorOfPublication | 717a0b7b-80e9-4e21-b320-6661506b0648 | |
relation.isAuthorOfPublication | 103f4509-876a-4bb4-b7ec-275bae82093a | |
relation.isAuthorOfPublication | 5a8fd625-08d7-448e-9224-9b2f1c16ef09 | |
relation.isAuthorOfPublication | 8a292654-a407-46e7-82c9-fa45b6487d27 | |
relation.isAuthorOfPublication.latestForDiscovery | 56cc70a6-13cb-4d22-9765-2b3a2b2f1536 |
Files
Original bundle
1 - 5 of 6
- Name:
- journal.pone.0078275.pdf
- Size:
- 1.25 MB
- Format:
- Adobe Portable Document Format
- Description:
- Article
No Thumbnail Available
- Name:
- Figure_S1.doc
- Size:
- 67.5 KB
- Format:
- Microsoft Word
- Description:
- Figure S1
No Thumbnail Available
- Name:
- Figure_S2.doc
- Size:
- 64.5 KB
- Format:
- Microsoft Word
- Description:
- Figure S2
No Thumbnail Available
- Name:
- Figure_S3.doc
- Size:
- 325 KB
- Format:
- Microsoft Word
- Description:
- Figure S3
No Thumbnail Available
- Name:
- Figure_S4.doc
- Size:
- 120.5 KB
- Format:
- Microsoft Word
- Description:
- Figure S4
License bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- license.txt
- Size:
- 1.89 KB
- Format:
- Item-specific license agreed upon to submission
- Description: