Quantitative PCR cell count estimates from samples of DNA extracted from seagrass wasting disease parasite, Labyrinthula zosterae from wasting disease mesocosm experiments at Bodega Marine Laboratory in July-Sept of 2015

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Date Created
2022-10-27
Location
Coastal New England
westlimit: -123.06572; southlimit: 38.31753; eastlimit: -123.06572; northlimit: 38.31753
DOI
10.26008/1912/bco-dmo.883055.1
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Keywords
climate change
host-parasite
microbiome
seagrass
wasting disease
Abstract
This data set includes quantitative PCR cell count estimates from samples of DNA extracted from seagrass wasting disease parasite, Labyrinthula zosterae, cultures of known cell concentrations run with and without DNA extracted from seagrass, Zostera marina, tissue samples to test for quantitative PCR signal inhibition. Seagrass tissue samples were collected as part of a mesocosm study at the Bodega Marine Laboratory examining the independent and interactive effects of warming, host genotypic identity, and host genotypic diversity on the prevalence and intensity of infections of seagrass by the wasting disease parasite L. zosterae. These data were plotted in Schenck et al (2022) as Figure S6. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/883055
Description
Dataset: Mesocosm warming experiment quantitative PCR inhibition controls
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Citation
Schenck, F., DuBois, K., Kardish, M., Stachowicz, J. J., & Hughes, A. R. (2022). Quantitative PCR cell count estimates from samples of DNA extracted from seagrass wasting disease parasite, Labyrinthula zosterae from wasting disease mesocosm experiments at Bodega Marine Laboratory in July-Sept of 2015 (Version 1) [Data set]. Biological and Chemical Oceanography Data Management Office (BCO-DMO). https://doi.org/10.26008/1912/BCO-DMO.883055.1
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