Diatom cultures used to generate DNA reference library from samples collected from sites in Alpena, Michigan and Palm Coast, Florida between July 2021 & 2022.
Diatom cultures used to generate DNA reference library from samples collected from sites in Alpena, Michigan and Palm Coast, Florida between July 2021 & 2022.
Date
2023-10-17
Authors
Hamsher, Sarah
Casamatta, Dale
Biddanda, Bopaiah
Casamatta, Dale
Biddanda, Bopaiah
Linked Authors
Alternative Title
Citable URI
Date Created
2023-10-17
Location
Middle Island, Lake Huron, Great Lakes N 45.19843°N, W083.32721°W
westlimit: -83.456; southlimit: 41.7679; eastlimit: -83.3245; northlimit: 45.1984
westlimit: -83.456; southlimit: 41.7679; eastlimit: -83.3245; northlimit: 45.1984
DOI
10.26008/1912/bco-dmo.911008.1
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Keywords
rbcL
Sanger sequencing
diatoms
Sanger sequencing
diatoms
Abstract
These data are the information for each of the cultures generated from samples collected from three sites in Alpena, Michigan, one site in Monroe, Michigan, and one site in Palm Coast, Florida. Data are for cultures sequenced using Sanger sequencing and include taxonomic identification, location and water parameter information from samples used to develop the cultures, and growth medium.
Each of these cultures was developed from high-sulfur, low-oxygen environments formed by underwater sinkholes and springs that create extreme habitats populated by microbial mat communities. Our study investigated previously undescribed diatom diversity in these habitats. Sequences from these cultures contribute to tying molecular data to morphologically identified isolates, providing a bridge between these two data types that can be used to improve metabarcoding analyses.
For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/911008
Description
Dataset: Diatom cultures used to generate DNA reference library
Embargo Date
Citation
Hamsher, S., Casamatta, D., & Biddanda, B. (2023). Diatom cultures used to generate DNA reference library from samples collected from sites in Alpena, Michigan and Palm Coast, Florida between July 2021 & 2022. (Version 1) [Data set]. Biological and Chemical Oceanography Data Management Office (BCO-DMO). https://doi.org/10.26008/1912/BCO-DMO.911008.1