The nucleoporin ALADIN regulates Aurora A localization to ensure robust mitotic spindle formation
The nucleoporin ALADIN regulates Aurora A localization to ensure robust mitotic spindle formation
Date
2015-08-05
Authors
Carvalhal, Sara
Ribeiro, Susana Abreu
Arocena, Miguel
Kasciukovic, Taciana
Temme, Achim
Koehler, Katrin
Huebner, Angela
Griffis, Eric R.
Ribeiro, Susana Abreu
Arocena, Miguel
Kasciukovic, Taciana
Temme, Achim
Koehler, Katrin
Huebner, Angela
Griffis, Eric R.
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10.1091/mbc.E15-02-0113
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Abstract
The formation of the mitotic spindle is a complex process that requires massive cellular reorganization. Regulation by mitotic kinases controls this entire process. One of these mitotic controllers is Aurora A kinase, which is itself highly regulated. In this study, we show that the nuclear pore protein ALADIN is a novel spatial regulator of Aurora A. Without ALADIN, Aurora A spreads from centrosomes onto spindle microtubules, which affects the distribution of a subset of microtubule regulators and slows spindle assembly and chromosome alignment. ALADIN interacts with inactive Aurora A and is recruited to the spindle pole after Aurora A inhibition. Of interest, mutations in ALADIN cause triple A syndrome. We find that some of the mitotic phenotypes that we observe after ALADIN depletion also occur in cells from triple A syndrome patients, which raises the possibility that mitotic errors may underlie part of the etiology of this syndrome.
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© The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Molecular Biology of the Cell 26 (2015): 3424-3438, doi:10.1091/mbc.E15-02-0113.
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Molecular Biology of the Cell 26 (2015): 3424-3438