The nucleoporin ALADIN regulates Aurora A localization to ensure robust mitotic spindle formation

dc.contributor.author Carvalhal, Sara
dc.contributor.author Ribeiro, Susana Abreu
dc.contributor.author Arocena, Miguel
dc.contributor.author Kasciukovic, Taciana
dc.contributor.author Temme, Achim
dc.contributor.author Koehler, Katrin
dc.contributor.author Huebner, Angela
dc.contributor.author Griffis, Eric R.
dc.date.accessioned 2015-12-11T20:15:03Z
dc.date.available 2015-12-11T20:15:03Z
dc.date.issued 2015-08-05
dc.description © The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Molecular Biology of the Cell 26 (2015): 3424-3438, doi:10.1091/mbc.E15-02-0113. en_US
dc.description.abstract The formation of the mitotic spindle is a complex process that requires massive cellular reorganization. Regulation by mitotic kinases controls this entire process. One of these mitotic controllers is Aurora A kinase, which is itself highly regulated. In this study, we show that the nuclear pore protein ALADIN is a novel spatial regulator of Aurora A. Without ALADIN, Aurora A spreads from centrosomes onto spindle microtubules, which affects the distribution of a subset of microtubule regulators and slows spindle assembly and chromosome alignment. ALADIN interacts with inactive Aurora A and is recruited to the spindle pole after Aurora A inhibition. Of interest, mutations in ALADIN cause triple A syndrome. We find that some of the mitotic phenotypes that we observe after ALADIN depletion also occur in cells from triple A syndrome patients, which raises the possibility that mitotic errors may underlie part of the etiology of this syndrome. en_US
dc.description.sponsorship This work was supported by a Wellcome Trust RCDF award (090064/Z/09/Z) to E.R.G., a Wellcome Trust Strategic award to the Centre for Gene Regulation and Expression (097945/B/11/Z), and a grant from the German Research Foundation to A.H. (HU895/5-1). S.C. was supported by a Biotechnology and Biological Sciences Research Council studentship. en_US
dc.format.mimetype application/pdf
dc.identifier.citation Molecular Biology of the Cell 26 (2015): 3424-3438 en_US
dc.identifier.doi 10.1091/mbc.E15-02-0113
dc.identifier.uri https://hdl.handle.net/1912/7686
dc.language.iso en_US en_US
dc.publisher American Society for Cell Biology en_US
dc.relation.uri https://doi.org/10.1091/mbc.E15-02-0113
dc.rights Attribution-NonCommercial-ShareAlike 3.0 Unported *
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0
dc.title The nucleoporin ALADIN regulates Aurora A localization to ensure robust mitotic spindle formation en_US
dc.type Article en_US
dspace.entity.type Publication
relation.isAuthorOfPublication 7fc4ecd8-2f23-45f0-858f-a1c4685eeb17
relation.isAuthorOfPublication 0fbf3469-01ef-4008-a229-f9fb7a42a380
relation.isAuthorOfPublication 76d0f00d-9808-4e3b-b347-23b3505b560d
relation.isAuthorOfPublication 3c36bf10-39ba-4472-9dbc-82cef8b997dc
relation.isAuthorOfPublication 21955057-7cc8-4978-ae05-2b37e4c56b80
relation.isAuthorOfPublication 53c3415b-1040-4e3c-ab54-7591fadd7d2d
relation.isAuthorOfPublication d6ee593d-73b8-441b-b68b-074b73df5f51
relation.isAuthorOfPublication dd91b806-c84c-4cbb-9ec8-338c6b800072
relation.isAuthorOfPublication.latestForDiscovery 7fc4ecd8-2f23-45f0-858f-a1c4685eeb17
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
Mol. Biol. Cell-2015-Carvalhal-3424-38.pdf
Size:
7.68 MB
Format:
Adobe Portable Document Format
Description:
Article
Thumbnail Image
Name:
CombinedSupMats.pdf
Size:
1.92 MB
Format:
Adobe Portable Document Format
Description:
Supplemental materials
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.89 KB
Format:
Item-specific license agreed upon to submission
Description:
Collections