Shimomura Osamu

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Shimomura
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Osamu
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  • Preprint
    A novel-type luciferin from Siberian luminous earthworm Fridericia heliota : structure elucidation by spectral studies and total synthesis
    ( 2014-02) Petushkov, Valentin N. ; Dubinnyi, Maxim A. ; Tsarkova, Aleksandra S. ; Rodionova, Natalja S. ; Baranov, Mikhail S. ; Kublitski, Vadim S. ; Shimomura, Osamu ; Yampolsky, Ilia V.
    We report structure elucidation and synthesis of the luciferin from the recently discovered luminous earthworm Fridericia heliota. This luciferin represents a key component of a novel ATP-dependent bioluminescence system. The UV, fluorescence, NMR and HRMS spectral studies were performed on 5 mkg of the isolated substance, and gave four isomeric structures, conforming with spectral data. These isomers were chemically synthesized and one of them was found to produce light in the reaction with a protein extract from Fridericia. The novel luciferin was found to have an unusual deeply modified peptidic nature, implying an unprecedented mechanism of action.
  • Article
    Obelin mutants as reporters in bioluminescent dual-analyte binding assay
    (RSC Publishing, 2012-11-29) Krasitskaya, Vasilisa V. ; Kudryavtsev, Alexander N. ; Shimomura, Osamu ; Frank, Ludmila A.
    Two obelin mutants distinctly different in the color of bioluminescence were successfully applied to simultaneous detection of two analytes in a single well. The flash-type signals were triggered by single injection of Ca2+ and were discriminated by the spectral and time resolutions. The technique was developed in a high-throughput format, and applied to simultaneous immunoassay of two gonadotropic hormones – luteinizing (lutropin or hLH) and follicle stimulating (hFSH) – in clinical sera, and to simultaneous detection of two gene allelic variants at single nucleotide polymorphism genotyping of the human F5 gene encoding factor V Leiden polymorphism 1691 G/A (R506Q). A considerable number of samples were investigated and the results obtained were in good correlation with those obtained by using traditional techniques.