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DatasetDissolved organic matter (DOM) and base-extracted particulate organic matter (BEPOM) collected from a plankton senescence experiment from water samples offshore of North Carolina(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2019-08-01) Osburn, Chris ; Bianchi, Thomas ; Schnetzer, Astrid ; Ziervogel, KaiA mixed assemblage of natural phytoplankton community collected from waters offshore of North Carolina were used to create planktonic dissolved organic matter (DOM) and particulate organic matter (POM). The latter was extracted with 0.1 M NaOH to create base-extracted POM (BEPOM). Methods are reported in Kinsey et al. (2018) and Osburn et al. (2019). The medium used was: Natural Assemblage 2x filtered North Atlantic Surface water with f/2 nutrients; Whole water experiment - unfiltered North Atlantic Surface water with f/20 nutrients Irradiance ~70-100 umol photon m-2 s-1 (cool white bulbs); on roller table; diel cycle, 19 °C For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/773802
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DatasetIchthyoplankton density and plankton displacement volume data collected using NOAA SEAMAP Neuston Plankton Nets on the NSF RAPID Plankton Cruises in the Northwestern Gulf of Mexico in 2017-2018(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-12-09) Geist, Simon ; McAskill, Shannan ; Paxton, Stormy ; Robinson, Kelly L. ; Stauffer, Beth ; Schnetzer, Astrid ; Zapfe, Glenn ; Bond, Pamela ; Drass, DeniceThis dataset includes ichthyoplankton density and plankton displacement volume data collected using NOAA SEAMAP Neuston Plankton Nets during three NSF RAPID Plankton cruises. RAPID Plankton Cruise 1 took place on R/V Point Sur, cruise number PS1813, from October 28-November 3, 2017. RAPID Plankton Cruise 2 took place on R/V Pelican, cruise number PE18-18, from January 6- 12, 2018. RAPID Plankton Cruise 3 took place on R/V Pelican, cruise number PE18-21, from March 19- 23, 2018. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/829239
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DatasetIchthyoplankton density and plankton displacement volume data collected using SEA-GEAR plankton ring nets on the NSF RAPID Plankton Cruises in the Northwestern Gulf of Mexico in 2017-2018(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-12-09) Geist, Simon ; McAskill, Shannan ; Paxton, Stormy ; Robinson, Kelly L. ; Stauffer, Beth ; Schnetzer, AstridThis dataset includes ichthyoplankton density and plankton displacement volume data collected using SEA-GEAR plankton ring nets during three NSF RAPID Plankton cruises. RAPID Plankton Cruise 1 took place on R/V Point Sur, cruise number PS1813, from October 28-November 3, 2017. RAPID Plankton Cruise 2 took place on R/V Pelican, cruise number PE18-18, from January 6- 12, 2018. RAPID Plankton Cruise 3 took place on R/V Pelican, cruise number PE18-21, from March 19- 23, 2018. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/829485
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DatasetCTD data collected on three NSF RAPID Plankton cruises on R/V Point Sur and R/V Pelican in the Gulf of Mexico during 2017-2018(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2021-08-05) Kurtay, Gulce ; Stauffer, Beth ; Robinson, Kelly L. ; Geist, Simon ; Schnetzer, AstridThis dataset includes processed CTD data collected during three NSF RAPID Plankton cruises. RAPID Plankton Cruise 1 took place on R/V Point Sur, cruise number PS1813, from October 28-November 3, 2017. RAPID Plankton Cruise 2 took place on R/V Pelican, cruise number PE18-18, from January 6- 12, 2018. RAPID Plankton Cruise 3 took place on R/V Pelican, cruise number PE18-21, from March 19- 23, 2018. Data were collected at 10 stations on each cruise. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/827969
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DatasetIchthyoplankton density and plankton displacement volume data collected using MOCNESS plankton nets on the NSF RAPID Plankton Cruises in the Northwestern Gulf of Mexico in 2017-2018(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-12-09) Geist, Simon ; McAskill, Shannan ; Paxton, Stormy ; Robinson, Kelly L. ; Stauffer, Beth ; Schnetzer, AstridThis dataset includes ichthyoplankton density and plankton displacement volume data collected using MOCNESS plankton nets (1x2m, equipped with 10 nets of 333um mesh widths) during three NSF RAPID Plankton cruises. RAPID Plankton Cruise 1 took place on R/V Point Sur, cruise number PS1813, from October 28-November 3, 2017. RAPID Plankton Cruise 2 took place on R/V Pelican, cruise number PE18-18, from January 6- 12, 2018. RAPID Plankton Cruise 3 took place on R/V Pelican, cruise number PE18-21, from March 19- 23, 2018. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/829545
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DatasetIchthyoplankton density and plankton displacement volume data collected using NOAA SEAMAP Bongo Plankton Nets on the NSF RAPID Plankton Cruises in the Northwestern Gulf of Mexico in 2017-2018(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-12-09) Geist, Simon ; McAskill, Shannan ; Paxton, Stormy ; Robinson, Kelly L. ; Stauffer, Beth ; Schnetzer, Astrid ; Zapfe, Glenn ; Bond, Pamela ; Drass, DeniceThis dataset includes ichthyoplankton density and plankton displacement volume data collected using NOAA SEAMAP Bongo Plankton Nets during three NSF RAPID Plankton cruises and one NOAA Plankton cruise. RAPID Plankton Cruise 1 took place on R/V Point Sur, cruise number PS1813, from October 28-November 3, 2017. RAPID Plankton Cruise 2 took place on R/V Pelican, cruise number PE18-18, from January 6- 12, 2018. RAPID Plankton Cruise 3 took place on R/V Pelican, cruise number PE18-21, from March 19- 23, 2018. The NOAA Plankton cruise took place on R/V Gordon Gunter, cruise number GU17-04, from September 04-30, 2017. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/829115
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PreprintDefining DNA-based operational taxonomic units for microbial-eukaryote ecology( 2009-06-19) Caron, David A. ; Countway, Peter D. ; Savai, Pratik ; Gast, Rebecca J. ; Schnetzer, Astrid ; Moorthi, Stefanie D. ; Dennett, Mark R. ; Moran, Dawn M. ; Jones, Adriane C.DNA sequence information has been increasingly used in ecological research on microbial eukaryotes. Sequence-based approaches have included studies of the total diversity of selected ecosystems, the autecology of ecologically relevant species, and the identification and enumeration of species of interest to human health. It is still uncommon, however, to delineate protistan species based on their genetic signatures. The reluctance to assign species-level designations based on DNA sequences is partly a consequence of the limited amount of sequence information presently available for many free-living microbial eukaryotes, and partly the problematic nature and debate surrounding the microbial species concept. Despite the difficulties inherent in assigning species names to DNA sequences, there is a growing need to attach meaning to the burgeoning amount of sequence information entering the literature, and a growing desire to apply this information in ecological studies. We describe a computer-based tool that assigns DNA sequences from environmental databases to operational taxonomic units at approximate species-level distinctions. The approach provides a practical method for ecological studies of microbial eukaryotes (primarily protists) by enabling semiautomated analysis of large numbers of samples spanning great taxonomic breadth. Derivation of the algorithm was based on an analysis of complete small subunit ribosomal RNA (18S) gene sequences and partial gene sequences obtained from GenBank for morphologically described protistan species. The program was tested using environmental 18S data sets from two oceanic ecosystems. A total of 388 operational taxonomic units were observed among 2,207 sequences obtained from samples collected in the western North Atlantic and eastern North Pacific.