Watanabe Shigeki

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Last Name
Watanabe
First Name
Shigeki
ORCID
0000-0001-7580-8141

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  • Article
    Asynchronous release sites align with NMDA receptors in mouse hippocampal synapses
    (Nature Research, 2021-01-29) Li, Shuo ; Raychaudhuri, Sumana ; Lee, Stephen Alexander ; Brockmann, Marisa M. ; Wang, Jing ; Kusick, Grant ; Prater, Christine ; Syed, Sarah ; Falahati, Hanieh ; Ramos, Raul ; Bartol, Tomas M. ; Hosy, Eric ; Watanabe, Shigeki
    Neurotransmitter is released synchronously and asynchronously following an action potential. Our recent study indicates that the release sites of these two phases are segregated within an active zone, with asynchronous release sites enriched near the center in mouse hippocampal synapses. Here we demonstrate that synchronous and asynchronous release sites are aligned with AMPA receptor and NMDA receptor clusters, respectively. Computational simulations indicate that this spatial and temporal arrangement of release can lead to maximal membrane depolarization through AMPA receptors, alleviating the pore-blocking magnesium leading to greater activation of NMDA receptors. Together, these results suggest that release sites are likely organized to activate NMDA receptors efficiently.
  • Article
    The synaptic vesicle cycle revisited: New insights into the modes and mechanisms
    (Society for Neuroscience, 2019-10-16) Chanaday, Natali L. ; Cousin, Michael A. ; Milosevic, Ira ; Watanabe, Shigeki ; Morgan, Jennifer R.
    Neurotransmission is sustained by endocytosis and refilling of synaptic vesicles (SVs) locally within the presynapse. Until recently, a consensus formed that after exocytosis, SVs are recovered by either fusion pore closure (kiss-and-run) or clathrin-mediated endocytosis directly from the plasma membrane. However, recent data have revealed that SV formation is more complex than previously envisaged. For example, two additional recycling pathways have been discovered, ultrafast endocytosis and activity-dependent bulk endocytosis, in which SVs are regenerated from the internalized membrane and synaptic endosomes. Furthermore, these diverse modes of endocytosis appear to influence both the molecular composition and subsequent physiological role of individual SVs. In addition, previously unknown complexity in SV refilling and reclustering has been revealed. This review presents a modern view of the SV life cycle and discusses how neuronal subtype, physiological temperature, and individual activity patterns can recruit different endocytic modes to generate new SVs and sculpt subsequent presynaptic performance.