Laws Edward

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Laws

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Series 2A-2: Multiple stressor experiments on T. pseudonana (CCMP1014) – photophysiology

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-11-30) Passow, Uta ; Laws, Edward ; Sweet, Julia

These experiments were designed to test the combined effects of temperatures and light intensity on the growth growth rate (mu) and photophysiology of the diatom Thalassiosira pseudonana CCMP 1014 in a multifactorial design. Experiments were conducted in artificial seawater supplemented with 5% sterilized seawater. Six temperatures (13.5°C, 20°C, 25°C, 29°C, 31°C, and 32.5°C), and eight light intensities (25, 50, 80, 115,190, 300, 400 and 600 µmol photons · m-2 · s-1 ) were tested during the course of these experiments. This dataset contains measurements of photophysiology using the Light curve (LC3) protocol of the Aquapen-C AP-C 100 fluorometer. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/829695
Series 2A-3: Multiple stressor experiments on T. pseudonana (CCMP1014) – Chlorophyll, particulate organic carbon and particulate organic nitrogen

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-11-30) Passow, Uta ; Laws, Edward ; Sweet, Julia

These experiments were designed to test the combined effects of temperatures and light intensity on the growth growth rate (mu) and photophysiology of the diatom Thalassiosira pseudonana CCMP 1014 in a multifactorial design. Experiments were conducted in artificial seawater supplemented with 5% sterilized seawater. Six temperatures (13.5°C, 20°C, 25°C, 29°C, 31°C, and 32.5°C), and eight light intensities (25, 50, 80, 115,190, 300, 400 and 600 umol photons · m-2 · s-1 ) were tested during the course of these experiments.This dataset contains measurements of extracted chlorophyll, particulate organic carbon (POC), and particulate organic nitrogen (PON) made over the course of the experiments. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/829707
Continuous culture studies of possible climate change effects: Thalassiosira pseudonana CCMP1335 growth in nitrate-limited and nutrient-replete cultures

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-09-30) Laws, Edward ; Passow, Uta

The marine diatom Thalassiosira pseudonana clone CCMP 1335 was grown in a continuous culture system on a 14:10 light-dark cycle under either nitrate-limited or nutrient-replete conditions, a photoperiod irradiance of either 50 or 300 micro-mol photons per square meter per second, partial pressures of either 400 or 1000 ppm CO2, and temperatures ranging from 5 to 32 degrees Celsius. Growth rates, photosynthetic rates, respiration rates, C:N ratios, C:Chlorophyll-a ratios, productivity indices, Fv/Fm ratios, and the initial slope and light-saturated asymptote of short-term photosynthesis-irradiance curves are reported. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/779368
Continuous culture studies of possible climate change effects: Synechococcus elongatus CCMP1629 growth in nitrate-limited and nutrient-replete cultures

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-09-30) Laws, Edward ; Passow, Uta

The marine cyanobacterium Synechococcus elongatus clone CCMP1629 was grown in a continuous culture system on a 14:10 light-dark cycle under either nitrate-limited or nutrient-replete conditions, a photoperiod irradiance of either 50 or 300 micro-mol photons per square meter per second, partial pressures of either 400 or 1000 ppm CO2, and temperatures ranging from 20 to 45 degrees Celsius. Growth rates, photosynthetic rates, respiration rates, C:N ratios, C:Chlorophyll-a ratios, productivity indices, Fv/Fm ratios, and the initial slope and light-saturated asymptote of short-term photosynthesis-irradiance curves are reported. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/811093
Series 4A: Multiple stressor experiments on Synechococcus elongatus (CCMP1629) – pH measurements

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-08-31) Passow, Uta ; Laws, Edward ; D'Souza, Nigel

The experiments were designed to test the combined effects of two CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the cyanobacteria Synechococcus elongatus CCMP1629 in a multifactorial design. This dataset contains measurements of pH made over the course of the experiments. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/806998
Series 4A: Multiple stressor experiments on Synechococcus elongatus (CCMP1629) – Dissolved Inorganic Carbon (DIC)

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-08-31) Passow, Uta ; Laws, Edward ; D'Souza, Nigel

The experiments were designed to test the combined effects of two CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the cyanobacteria Synechococcus elongatus CCMP1629 in a multifactorial design. This dataset contains measurements of Dissolved Inorganic Carbon (DIC) made over the course of the experiments. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/807010
Series 2A-1: Multiple stressor experiments on T. pseudonana (CCMP1014) – cell abundance and cell size in experiments

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-11-30) Passow, Uta ; Laws, Edward ; Sweet, Julia

These experiments were designed to test the combined effects of temperatures and light intensity on the growth growth rate (mu) and photophysiology of the diatom Thalassiosira pseudonana CCMP 1014 in a multifactorial design. Experiments were conducted in artificial seawater supplemented with 5% sterilized seawater. Six temperatures (13.5°C, 20°C, 25°C, 29°C, 31°C, and 32.5°C), and eight light intensities (25, 50, 80, 115,190, 300, 400 and 600 umol photons · m-2 · s-1 ) were tested during the course of these experiments. This dataset contains measurements of cell abundances and cell size expressed as forward scatter (FSC) as well as in equivalent spherical diameter (ESD) in microns. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/829675
Series 1B-3: Multiple stressor experiments on T. pseudonana (CCMP1335) – computed data from the LC3 protocol for samples at 4 temperatures, 15-26C

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-11-30) Passow, Uta ; Laws, Edward ; Sweet, Julia

Four follow-up experiments on the combined effect of light and temperature changes on the growth rate (mu) and photophysiology of Thalassiosira pseudonana CCMP 1335 were conducted to supplement / repeat series 1A experiments. This was necessary because doubt existed regarding the growth during 1A experiments. 1A experiments were conducted in artificial seawater. 1B experiments were conducted in artificial seawater supplemented with 5% sterilized seawater. The experiments were designed to test the combined effects of four temperatures, and eight light intensities on the growth and photophysiology of the diatom T. pseudonana CCMP1335 in a multifactorial design. This dataset contains measurements of photophysiology using the Light curve (LC3) protocol of the Aquapen-C AP-C 100 fluorometer. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/829009
Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - Chlorophyll, particulate organic carbon and particulate organic nitrogen.

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-06-29) Passow, Uta ; Laws, Edward ; D'Souza, Nigel

The experiments were designed to test the combined effects of CO2, temperatures, and light on growth and photophysiology of the diatom T. pseudonana CCMP1014 in a multifactorial design. This dataset contains measurements of extracted chlorophyll, particulate organic carbon (POC), and particulate organic nitrogen (PON) made over the course of the experiments. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/771594
Series 4A: Multiple stressor experiments on the cyanobacteria Synechococcus elongatus CCMP1629 - raw fluorescence readings for photophysiology computations

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-08-31) Passow, Uta ; Laws, Edward ; D'Souza, Nigel

The experiments were designed to test the combined effects of two CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the cyanobacteria S. elongatus CCMP1629 in a multifactorial design. This dataset contains raw fluorescence measurements for computing photophysiology using the Light curve (LC3) protocol of the Aquapen-C AP-C 100 fluorometer. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/808174
Series 4A: Multiple stressor experiments on Synechococcus elongatus (CCMP1629) – nutrients

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-08-31) Passow, Uta ; Laws, Edward ; D'Souza, Nigel

The experiments were designed to test the combined effects of two CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the cyanobacteria Synechococcus elongatus CCMP1629 in a multifactorial design. This dataset contains measurements of nutrients (phosphate, nitrate, and nitrate + nitrite) made over the course of the experiments. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/807026
Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) – cell abundance by flow cytometry

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-06-29) Passow, Uta ; Laws, Edward ; D'Souza, Nigel

The experiments were designed to test the combined effects of three CO2 concentrations, four temperatures, and three light intensities on growth of the diatom T. pseudonana CCMP1014 in a multifactorial design. This dataset contains measurements of cell abundances measured by forward scatter. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/771421
Experimental results of nitrate-limited or nitrate-replete continuous culture studies of Thalassiosira pseudonana at 5 temperatures with either high or low pCO2 and irradiance

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-09-30) Laws, Edward ; Passow, Uta

Thalassiosira pseudonanana were grown in nitrate limited culture at five temperatures, 400 umol photons m–2 s–1, and 1000 ppm CO2 and irradiance levels of 50 and 300 umol photons m-2 s-1. Growth rates, photosynthetic rates, respiration rates, C:N ratio, C:Chlorophyll-a ratio, maximum quantum yield are reported. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/746398
Series 1B-4: Multiple stressor experiments on T. pseudonana (CCMP1335) – Chlorophyll, particulate organic carbon and particulate organic nitrogen

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-11-30) Passow, Uta ; Laws, Edward ; Sweet, Julia

Four follow-up experiments on the combined effect of light and temperature changes on the growth rate (mu) and photophysiology of Thalassiosira pseudonana CCMP 1335 were conducted to supplement / repeat series 1A experiments. This was necessary because doubt existed regarding the growth during 1A experiments. 1A experiments were conducted in artificial seawater. 1B experiments were conducted in artificial seawater supplemented with 5% sterilized seawater. The experiments were designed to test the combined effects of four temperatures, and eight light intensities on the growth and photophysiology of the diatom T. pseudonana CCMP1335 in a multifactorial design.This dataset contains measurements of extracted chlorophyll, particulate organic carbon (POC), and particulate organic nitrogen (PON) made over the course of the experiments. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/829025
Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - Phosphate, silicate, and nitrate plus nitrite measurements

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-06-29) Passow, Uta ; Laws, Edward ; D'Souza, Nigel

The experiments were designed to test the combined effects of three CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the diatom T. pseudonana CCMP1014 in a multifactorial design. This dataset contains measurements of nutrients (phosphate, silicate, and nitrate plus nitrite) made over the course of the experiments. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/771370
Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - photophysiology measurements

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-06-29) Passow, Uta ; Laws, Edward ; D'Souza, Nigel

The experiments were designed to test the combined effects of CO2, temperatures, and light on the growth of the diatom T. pseudonana CCMP1014 in a multifactorial design. This dataset contains measurements of photophysiology using the Light curve (LC3) protocol of the Aquapen-C AP-C 100 fluorometer. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/771461
Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) – pH measurements

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-06-29) Passow, Uta ; Laws, Edward ; D'Souza, Nigel

The experiments were designed to test the combined effects of three CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the diatom T. pseudonana CCMP1014 in a multifactorial design. This dataset contains measurements of pH made over the course of the experiments. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/771304
Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - cell size estimated by forward scatter from flow cytometry

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-06-29) Passow, Uta ; Laws, Edward ; D'Souza, Nigel

The experiments were designed to test the combined effects of three CO2 concentrations, four temperatures, and three light intensities on growth of the diatom T. pseudonana CCMP1014 in a multifactorial design. This dataset contains measurements of cell size expressed as forward scatter as well as in equivalent spherical diameter (ESD) in microns. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/771448
Series 1B-1: Multiple stressor experiments on T. pseudonana (CCMP1335) – pH, Dissolved Inorganic carbon (DIC), and Macronutrient concentrations in experiments

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-11-30) Passow, Uta ; Laws, Edward ; Sweet, Julia

Four follow-up experiments on the combined effect of light and temperature changes on the growth rate (µ) and photophysiology of Thalassiosira pseudonana CCMP 1335 were conducted to supplement / repeat series 1A experiments. This was necessary because doubt existed regarding the growth during 1A experiments. 1A experiments were conducted in artificial seawater. 1B experiments were conducted in artificial seawater supplemented with 5% sterilized seawater. This data set contains the carbonate system parameters.The experiments were designed to test the combined effects of four temperatures, and eight light intensities on the growth and photophysiology of the diatom T. pseudonana CCMP1335 in a multifactorial design. This dataset contains measurements of pH, DIC and macronutrient (N, P and Si) concentrations made over the course of the experiments For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/828910
Series 4A: Multiple stressor experiments on the cyanobacteria Synechococcus elongatus CCMP1629 – cell abundance and size by flow cytometry

(Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-08-31) Passow, Uta ; Laws, Edward ; D'Souza, Nigel

The experiments were designed to test the combined effects of two CO2 concentrations, four temperatures, and three light intensities on growth and photophysiology of the cyanobacteria Synechococcus elongatus CCMP1629 in a multifactorial design. This dataset contains measurements of cell abundances and cell size expressed as forward scatter (FSC) as well as in equivalent spherical diameter (ESD) in um. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/807327

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