Caprara Salvatore

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Caprara
First Name
Salvatore
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  • Dataset
    Iron concentrations of phage from experiments of iron-labelled E. coli infected with T4 and T5 bacteriophage, 2018 and 2019.
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-01-27) Breitbart, Mya ; Buck, Kristen N. ; Bonnain, Chelsea ; Caprara, Salvatore
    This data was collected as part of a study investigating the source of iron to bacteriophage (phage for short, or viruses that infect and kill bacteria) progeny. Evidence from a phage that infects E. coli shows iron incorporated into the tail fiber structure. This study aims at identifying whether the source of the iron is environmental or bacterially derived. E. coli bacterial cultures were grown in minimal media spiked with 10 µM 57FeSO4 then infected with phage T4 or T5. The phages were purified by methods of centrifugation, filtration, density-dependent ultracentrifugation, and dialyzing. The resulting phage fractions were quantified by SYBR epifluorescence microscopy and metal concentrations were measured on an ELEMENT XR ICP-MS. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/757485
  • Dataset
    57Fe Wall Loss Experiment data collected as part of a method development study investigating the precipitation and wall loss of labeled 57Fe when added to M9 Minimal Media
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-02-11) Breitbart, Mya ; Buck, Kristen N. ; Bonnain, Chelsea ; Caprara, Salvatore
    This data was collected as part of a method development study investigating the precipitation and wall loss of labeled ⁵⁷Fe when added to M9 Minimal Media, which is used to grow the model bacterial species Escherichia coli. The bulk media was prepared with the same components and a portion was treated with Chelex-100 to remove metals, while the other portion remained un-chelexed. Half of each treatment was spiked with labeled ⁵⁷Fe and either 0.2 μm or 0.02 μm filtered for comparison of the dissolved and soluble fractions. The ⁵⁷Fe content was monitored over four time points for one week in a shaking incubator, under the same conditions used to culture E. coli for labeling experiments. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/732864
  • Dataset
    Dissolved iron speciation results from 2019 Bermuda Atlantic Iron Time-series (BAIT) cruises EN631, AE1909, AE1921, AE1930 in the Western Subtropical North Atlantic Gyre from March to November 2019 (BAIT Project)
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2023-02-03) Buck, Kristen ; Caprara, Salvatore
    Dissolved iron-binding organic ligand concentrations and conditional stability constants from GO-FLO water column samples collected in 2019 during four Bermuda Atlantic Iron Time-serires (BAIT) cruises in the Bermuda Atlantic Time-series Study (BATS) region. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/869081
  • Article
    An operational overview of the EXport Processes in the Ocean from RemoTe Sensing (EXPORTS) Northeast Pacific field deployment
    (University of California Press, 2021-07-07) Siegel, David A. ; Cetinić, Ivona ; Graff, Jason R. ; Lee, Craig M. ; Nelson, Norman B. ; Perry, Mary J. ; Soto Ramos, Inia ; Steinberg, Deborah K. ; Buesseler, Ken O. ; Hamme, Roberta C. ; Fassbender, Andrea ; Nicholson, David P. ; Omand, Melissa M. ; Robert, Marie ; Thompson, Andrew F. ; Amaral, Vinicius ; Behrenfeld, Michael J. ; Benitez-Nelson, Claudia R. ; Bisson, Kelsey ; Boss, Emmanuel S. ; Boyd, Philip ; Brzezinski, Mark A. ; Buck, Kristen N. ; Burd, Adrian B. ; Burns, Shannon ; Caprara, Salvatore ; Carlson, Craig A. ; Cassar, Nicolas ; Close, Hilary G. ; D'Asaro, Eric A. ; Durkin, Colleen A. ; Erickson, Zachary K. ; Estapa, Margaret L. ; Fields, Erik ; Fox, James ; Freeman, Scott ; Gifford, Scott M. ; Gong, Weida ; Gray, Deric ; Guidi, Lionel ; Haëntjens, Nils ; Halsey, Kim ; Huot, Yannick ; Hansell, Dennis A. ; Jenkins, Bethany D. ; Karp-Boss, Lee ; Kramer, Sasha J. ; Lam, Phoebe J. ; Lee, Jong-Mi ; Maas, Amy E. ; Marchal, Olivier ; Marchetti, Adrian ; McDonnell, Andrew M. P. ; McNair, Heather ; Menden-Deuer, Susanne ; Morison, Francoise ; Niebergall, Alexandria K. ; Passow, Uta ; Popp, Brian N. ; Potvin, Geneviève ; Resplandy, Laure ; Roca-Martí, Montserrat ; Roesler, Collin S. ; Rynearson, Tatiana A. ; Traylor, Shawnee ; Santoro, Alyson E. ; Seraphin, Kanesa ; Sosik, Heidi M. ; Stamieszkin, Karen ; Stephens, Brandon M. ; Tang, Weiyi ; Van Mooy, Benjamin ; Xiong, Yuanheng ; Zhang, Xiaodong
    The goal of the EXport Processes in the Ocean from RemoTe Sensing (EXPORTS) field campaign is to develop a predictive understanding of the export, fate, and carbon cycle impacts of global ocean net primary production. To accomplish this goal, observations of export flux pathways, plankton community composition, food web processes, and optical, physical, and biogeochemical (BGC) properties are needed over a range of ecosystem states. Here we introduce the first EXPORTS field deployment to Ocean Station Papa in the Northeast Pacific Ocean during summer of 2018, providing context for other papers in this special collection. The experiment was conducted with two ships: a Process Ship, focused on ecological rates, BGC fluxes, temporal changes in food web, and BGC and optical properties, that followed an instrumented Lagrangian float; and a Survey Ship that sampled BGC and optical properties in spatial patterns around the Process Ship. An array of autonomous underwater assets provided measurements over a range of spatial and temporal scales, and partnering programs and remote sensing observations provided additional observational context. The oceanographic setting was typical of late-summer conditions at Ocean Station Papa: a shallow mixed layer, strong vertical and weak horizontal gradients in hydrographic properties, sluggish sub-inertial currents, elevated macronutrient concentrations and low phytoplankton abundances. Although nutrient concentrations were consistent with previous observations, mixed layer chlorophyll was lower than typically observed, resulting in a deeper euphotic zone. Analyses of surface layer temperature and salinity found three distinct surface water types, allowing for diagnosis of whether observed changes were spatial or temporal. The 2018 EXPORTS field deployment is among the most comprehensive biological pump studies ever conducted. A second deployment to the North Atlantic Ocean occurred in spring 2021, which will be followed by focused work on data synthesis and modeling using the entire EXPORTS data set.