Kubanek Julia

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Kubanek
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Julia
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  • Dataset
    Juvenile oyster shell strength measurements from predator cue bioassay experiments with treatments including blue crab urine, homarine, and trigonelline conducted at Dauphin Island Sea Lab, Dauphin Island, AL between June and August of 2020
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2022-12-01) Roney, Sarah Hope ; Cepeda, Marisa ; Belgrad, Benjamin A. ; Smee, Delbert Lee ; Kubanek, Julia ; Weissburg, Marc
    These data include measurements of juvenile oyster shell strength from predator cue bioassay experiments conducted at Dauphin Island Sea Lab, Dauphin Island, AL between June and August of 2020. Study description: Homarine and trigonelline are two blue crab urine metabolites that cause mud crabs to seek refuge, but it is unknown whether these molecules influence other species. In the current study, homarine, trigonelline, and blue crab urine of animals fed conspecific and heterospecific diets were tested on juvenile oysters to ascertain if the same molecules known to alter mud crab behavior also affect oyster morphology. Juvenile oysters were exposed to chemicals for roughly 6 weeks and their shell strength (N) was measured and standardized to the size of the animals (mm) as a proxy for understanding this defense. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/883945
  • Article
    Biochemical warfare on the reef : the role of glutathione transferases in consumer tolerance of dietary prostaglandins
    (Public Library of Science, 2010-01-06) Whalen, Kristen E. ; Lane, Amy L. ; Kubanek, Julia ; Hahn, Mark E.
    Despite the profound variation among marine consumers in tolerance for allelochemically-rich foods, few studies have examined the biochemical adaptations underlying diet choice. Here we examine the role of glutathione S-transferases (GSTs) in the detoxification of dietary allelochemicals in the digestive gland of the predatory gastropod Cyphoma gibbosum, a generalist consumer of gorgonian corals. Controlled laboratory feeding experiments were used to investigate the influence of gorgonian diet on Cyphoma GST activity and isoform expression. Gorgonian extracts and semi-purified fractions were also screened to identify inhibitors and possible substrates of Cyphoma GSTs. In addition, we investigated the inhibitory properties of prostaglandins (PGs) structurally similar to antipredatory PGs found in high concentrations in the Caribbean gorgonian Plexaura homomalla. Cyphoma GST subunit composition was invariant and activity was constitutively high regardless of gorgonian diet. Bioassay-guided fractionation of gorgonian extracts revealed that moderately hydrophobic fractions from all eight gorgonian species examined contained putative GST substrates/inhibitors. LC-MS and NMR spectral analysis of the most inhibitory fraction from P. homomalla subsequently identified prostaglandin A2 (PGA2) as the dominant component. A similar screening of commercially available prostaglandins in series A, E, and F revealed that those prostaglandins most abundant in gorgonian tissues (e.g., PGA2) were also the most potent inhibitors. In vivo estimates of PGA2 concentration in digestive gland tissues calculated from snail grazing rates revealed that Cyphoma GSTs would be saturated with respect to PGA2 and operating at or near physiological capacity. The high, constitutive activity of Cyphoma GSTs is likely necessitated by the ubiquitous presence of GST substrates and/or inhibitors in this consumer's gorgonian diet. This generalist's GSTs may operate as ‘all-purpose’ detoxification enzymes, capable of conjugating or sequestering a broad range of lipophilic gorgonian compounds, thereby allowing this predator to exploit a range of chemically-defended prey, resulting in a competitive dietary advantage for this species.
  • Dataset
    Juvenile oyster shell strength measurements from a dose response assay of chemical cues homarine and trigonelline conducted at Dauphin Island Sea Lab, Dauphin Island, AL in June - August 2021
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2022-12-01) Roney, Sarah Hope ; Cepeda, Marisa ; Belgrad, Benjamin A. ; Smee, Delbert Lee ; Kubanek, Julia ; Weissburg, Marc
    These data include measurements of juvenile oyster shell strength from a dose response experiment conducted at Dauphin Island Sea Lab, Dauphin Island, AL in June - August of 2021. Study description: Homarine and trigonelline are two blue crab urine metabolites that cause juvenile oysters to strengthen their shells are a defensive response. We evaluated the dose dependency of this response with a dose-response experiment where homarine and trigonelline concentrations (of each individual chemical and a combination of the two) spanned 5 log half-steps. Juvenile oysters were exposed to chemicals for 8 weeks and their shell strength (N) was measured and standardized to the size of the animals (mm) as a proxy for understanding this defense. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/883999
  • Dataset
    Juvenile oyster shell strength measurements from a dose response experiment with an array of blue crab urine concentrations conducted at Dauphin Island Sea Lab, Dauphin Island, AL in August - Oct 2022
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2022-12-01) Roney, Sarah Hope ; Cepeda, Marisa ; Belgrad, Benjamin A. ; Smee, Delbert Lee ; Kubanek, Julia ; Weissburg, Marc
    These data include measurements of juvenile oyster shell strength from a dose response experiment of varying blue crab urine concentrations conducted at Dauphin Island Sea Lab, Dauphin Island, AL in August - Oct 2022. Study description: Metabolites from blue crab urine are known to cause shell strengthening in juvenile oysters as a defensive response. Previous studies have identified several bioactive molecules in urine that induce this response in oysters, but others have yet to be identified. In the current study, an array of concentrations of blue crab urine was used to treat oyster juveniles in order to assess the dose-dependency of this response. Oysters were exposed to urine treatments for 8 weeks and their shell strength (N) was measured and standardized to the size of the animals (mm) as a proxy for understanding this defense. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/884015