Fiore Cara L.

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Fiore
First Name
Cara L.
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  • Dataset
    Isotopic analysis of ¹³C and ¹⁵N for sponges, coral, and zooxanthellae (family Symbiodiniaceae) used in a 'pulse-chase' experiment to examine the uptake of sponge-derived nutrients by the coral holobiont
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2023-02-24) Reigel, Alicia M. ; Easson, Cole G. ; Apprill, Amy ; Freeman, Christopher J. ; Bartley, Michaela M. ; Fiore, Cara L.
    These are raw data from isotopic analysis of ¹³C and ¹⁵N for sponges, coral, and zooxanthellae (family Symbiodiniaceae) used in a 'pulse-chase' experiment to examine the uptake of sponge-derived nutrients by the coral holobiont. Coral were collected from the Florida Keys National Marine Sanctuary and the experiments were carried out at the Climate and Acidification Ocean Simulator (CAOS) at Mote Marine Laboratory at Summerland Key, Florida, USA. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/889857
  • Preprint
    Release of ecologically relevant metabolites by the cyanobacterium Synechococcus elongatus CCMP 1631
    ( 2015-04-30) Fiore, Cara L. ; Longnecker, Krista ; Kido Soule, Melissa C. ; Kujawinski, Elizabeth B.
    Photoautotrophic plankton in the surface ocean release organic compounds that fuel secondary production by heterotrophic bacteria. Here we show that an abundant marine cyanobacterium, Synechococcus elongatus, contributes a variety of nitrogen-rich and sulfur-containing compounds to dissolved organic matter. A combination of targeted and untargeted metabolomics and genomic tools was used to characterize the intracellular and extracellular metabolites of S. elongatus. Aromatic compounds such as 4-hydroxybenzoic acid and phenylalanine, as well as nucleosides (e.g., thymidine, 5’-methylthioadenosine, xanthosine), the organosulfur compound 3-mercaptopropionate, and the plant auxin indole 3-acetic acid, were released by S. elongatus at multiple time points during its growth. Further, the amino acid kynurenine was found to accumulate in the media even though it was not present in the predicted metabolome of S. elongatus. This indicates that some metabolites, including those not predicted by an organism’s genome, are likely excreted into the environment as waste; however, these molecules may have broader ecological relevance if they are labile to nearby microbes. The compounds described herein provide excellent targets for quantitative analysis in field settings to assess the source and lability of dissolved organic matter in situ.
  • Article
    A phosphate starvation response gene (psr1-like) is present and expressed in Micromonas pusilla and other marine algae
    (Inter Research, 2021-02-25) Fiore, Cara L. ; Alexander, Harriet ; Kido Soule, Melissa C. ; Kujawinski, Elizabeth B.
    Phosphorus (P) limits primary production in regions of the surface ocean, and many plankton species exhibit specific physiological responses to P deficiency. The metabolic response of Micromonas pusilla, an ecologically relevant marine photoautotroph, to P deficiency was investigated using metabolomics and comparative genomics. The concentrations of some intracellular metabolites were elevated in the P-deficient cells (e.g. xanthine, inosine), and genes involved in the associated metabolic pathways shared a predicted conserved amino acid motif in the non-coding regions of each gene. The presence of the conserved motif suggests that these genes may be co-regulated, and the motif may constitute a regulatory element for binding a transcription factor, specifically that of Psr1 (phosphate starvation response). A putative phosphate starvation response gene ( psr1-like) was identified in M. pusilla with homology to well characterized psr1/ phr1 genes in algae and plants, respectively. This gene appears to be present and expressed in other marine algal taxa (e.g. Emiliania huxleyi) in field sites that are chronically P limited. Results from the present study have implications for understanding phytoplankton taxon-specific roles in mediating P cycling in the ocean.
  • Article
    Sponge exhalent seawater contains a unique chemical profile of dissolved organic matter
    (PeerJ, 2017-01-11) Fiore, Cara L. ; Freeman, Christopher J. ; Kujawinski, Elizabeth B.
    Sponges are efficient filter feeders, removing significant portions of particulate and dissolved organic matter (POM, DOM) from the water column. While the assimilation and respiration of POM and DOM by sponges and their abundant microbial symbiont communities have received much attention, there is virtually no information on the impact of sponge holobiont metabolism on the composition of DOM at a molecular-level. We applied untargeted and targeted metabolomics techniques to characterize DOM in seawater samples prior to entering the sponge (inhalant reef water), in samples exiting the sponge (exhalent seawater), and in samples collected just outside the reef area (off reef seawater). Samples were collected from two sponge species, Ircinia campana and Spheciospongia vesparium, on a near-shore hard bottom reef in the Florida Keys. Metabolic profiles generated from untargeted metabolomics analysis indicated that many more compounds were enhanced in the exhalent samples than in the inhalant samples. Targeted metabolomics analysis revealed differences in diversity and concentration of metabolites between exhalent and off reef seawater. For example, most of the nucleosides were enriched in the exhalent seawater, while the aromatic amino acids, caffeine and the nucleoside xanthosine were elevated in the off reef water samples. Although the metabolic profile of the exhalent seawater was unique, the impact of sponge metabolism on the overall reef DOM profile was spatially limited in our study. There were also no significant differences in the metabolic profiles of exhalent water between the two sponge species, potentially indicating that there is a characteristic DOM profile in the exhalent seawater of Caribbean sponges. Additional work is needed to determine whether the impact of sponge DOM is greater in habitats with higher sponge cover and diversity. This work provides the first insight into the molecular-level impact of sponge holobiont metabolism on reef DOM and establishes a foundation for future experimental studies addressing the influence of sponge-derived DOM on chemical and ecological processes in coral reef ecosystems.
  • Dataset
    Coral fragment surface area calculations utilizing two methods (tin foil and Image J) and corresponding zooxanthellae count data
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2022-10-03) Reigel, Alicia M. ; Easson, Cole G. ; Apprill, Amy ; Freeman, Christopher J. ; Bartley, Michaela M. ; Fiore, Cara L.
    This dataset presents coral fragment surface area calculations utilizing two methods (tin foil and Image J) and corresponding zooxanthellae count data. Three coral species were utilized in this experiment: the octocoral, Eunicea flexuosa, and two hard coral species, Acropora cervicornis and Orbicella faveolata. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/880711