Payne Diana

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Payne
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Diana
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  • Dataset
    Salp specimen log for genomic and transcriptomic study collected from ARSV Laurence M. Gould, Umitaka-Maru, R/V Polarstern LMG1110, UM-08-09, ANT-XXVII-2 in the Southern Ocean from 2009-2011 (Salp_Antarctic project)
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2018-02-23) Bucklin, Ann ; O'Neill, Rachel J. ; Payne, Diana
    This dataset is a sample log of the Salpa thompsoni specimens used for genomic and transcriptomic analysis. Collections were from three cruises in the Southern Ocean. Collections were made in Summer of 2009 during a cruise of the Umitaka-Maru (UM-08-09) from off-shelf sites in the Indian Sector. During cruises of the ARSV Laurence M. Gould (LMG-1110) and R/V Polarstern (ANT-XXVII-2) samples were collected from on- and off-shelf regions of the Western Antarctic Peninsula Region and in Bransfield Strait during Spring and Summer 2011, respectively. Reported parameters include cruise id, region, season and area, location, number of samples collected, temperature, salinity, sampled depth range, and the depth of the water at the collection site. For a complete list of measurements, refer to the supplemental document 'Field_names.pdf', and a full dataset description is included in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: http://www.bco-dmo.org/dataset/672600
  • Dataset
    Antarctic salp genome and RNAseq transcriptome from ARSV Laurence M. Gould, Umitaka-Maru, R/V Polarstern LMG1110, UM-08-09, ANT-XXVII-2 in the Southern Ocean from 2009-2011 (Salp_Antarctic project)
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2018-02-23) Bucklin, Ann ; O'Neill, Rachel J. ; Payne, Diana
    A preliminary genome sequence and complete reference transcriptome have been assembled for the Southern Ocean salp, Salpa thompsoni (Urochordata, Thaliacea). The reference transcriptome contains 216,931 sequences; 41,210 (18%) were associated with predicted, hypothetical, or known proteins; 13,058 (6%) were mapped and annotated. Whole-transcriptome (RNA-seq) analysis of 39 samples collected during austral spring and summer 2011 in the WAP, and in summer 2009 in the Indian Sector revealed clustering of samples by regions, seasons, and areas (Bray-Curtis similarity). Spring versus summer samples showed significant differential expression of 77 genes associated with environmental stress response and 51 genes associated with sexual reproduction (paired t-tests, p<0.05). Gene Ontology (GO) term enrichment analysis identified 41 GO terms responsible for spring versus summer differences, including 156 genes associated with translation (i.e., protein synthesis). The genome sequence of 318,767,936 bp covers >50% of the estimated 602 MB (±173 MB) genome size for S. thompsoni, with >50% (16,823) of sequences showing significant homology to known proteins and ~38% (12,151) of the total protein predictions associated with Gene Ontology functional information. A total of 109,958 SNP variants and 9,782 indel predictions were generated, serving as a resource for future phylogenomic and population genomic studies. Salpa thompsoni exhibits rapid rates of evolution (>1.5 times that observed for vertebrates) typical of other urochordates examined. An initial survey of small RNAs revealed the presence of known, conserved miRNAs, as well as novel miRNA genes; unique piRNAs; and mature miRNA signatures for varying developmental stages. For a complete list of measurements, refer to the supplemental document 'Field_names.pdf', and a full dataset description is included in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: http://www.bco-dmo.org/dataset/675040