Rhodes Heather J.

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Last Name
Rhodes
First Name
Heather J.
ORCID
0000-0003-0333-683X

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  • Article
    Electrophysiological and motor responses to chemosensory stimuli in isolated cephalopod arms
    (University of Chicago Press, 2020-02-17) Fouke, Kaitlyn E. ; Rhodes, Heather J.
    While there is behavioral and anatomical evidence that coleoid cephalopods use their arms to “taste” substances in the environment, the neurophysiology of chemosensation has been largely unexamined. The range and sensitivity of detectable chemosensory stimuli, and the processing of chemosensory information, are unknown. To begin to address these issues, we developed a technique for recording neurophysiological responses from isolated arms, allowing us to test responses to biologically relevant stimuli. We tested arms from both a pelagic species (Doryteuthis pealeii) and a benthic species (Octopus bimaculoides) by attaching a suction electrode to the axial nerve cord to record neural activity in response to chemical stimuli. Doryteuthis pealeii arms showed anecdotal responses to some stimuli but generally did not tolerate the preparation; tissue was nonviable within minutes ex vivo. Octopus bimaculoides arms were used successfully, with tissue remaining healthy and responsive for several hours. Arms responded strongly to fish skin extract, glycine, methionine, and conspecific skin extract but not to cephalopod ink or seawater controls. Motor responses were also observed in response to detected stimuli. These results suggest that chemosensory receptor cells on O. bimaculoides arms were able to detect environmentally relevant chemicals and drive local motor responses within the arm. Further exploration of potential chemical stimuli for O. bimaculoides arms, as well as investigations into the neural processing within the arm, could enhance our understanding of how this species uses its arms to explore its environment. While not successful in D. pealeii, this technique could be attempted with other cephalopod species, as comparative questions remain of interest.
  • Article
    Electrophysiological responses to conspecific odorants in Xenopus laevis show potential for chemical signaling
    (Public Library of Science, 2022-09-02) Rhodes, Heather J ; Amo, Melanie
    The fully aquatic African clawed frog, Xenopus laevis, has an unusual and highly adapted nose that allows it to separately sample both airborne and waterborne stimuli. The function of the adult water nose has received little study, despite the fact that it is quite likely to receive information about conspecifics through secretions released into the water and could aid the frog in making decisions about social and reproductive behaviors. To assess the potential for chemical communication in this species, we developed an in situ electroolfactogram preparation and tested the olfactory responses of adult males to cloacal fluids and skin secretions from male and female conspecifics. We found robust olfactory responses to all conspecific stimuli, with greatest sensitivity to female cloacal fluids. These results open the door to further testing to identify compounds within cloacal fluids and skin secretions that are driving these responses and examine behavioral responses to those compounds. Understanding the role of chemical communication in social and reproductive behaviors may add to our rich understanding of vocal communication to create a more complete picture of social behavior in this species.