Eliceiri Kevin

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Last Name
Eliceiri
First Name
Kevin
ORCID
0000-0001-8678-670X

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  • Article
    Quantification of collagen organization in histopathology samples using liquid crystal based polarization microscopy
    (The Optical Society, 2017-08-29) Keikhosravi, Adib ; Liu, Yuming ; Drifka, Cole ; Woo, Kaitlin M. ; Verma, Amitabh ; Oldenbourg, Rudolf ; Eliceiri, Kevin
    A number of histopathology studies have utilized the label free microscopy method of Second Harmonic Generation (SHG) to investigate collagen organization in disease onset and progression. Here we explored an alternative label free imaging approach, LC-PolScope that is based on liquid crystal based polarized light imaging. We demonstrated that this more accessible technology has the ability to visualize all fibers of interest and has a good to excellent correlation between SHG and LC-PolScope measurements in fibrillar collagen orientation and alignment. This study supports that LC-PolScope is a viable alternative to SHG for label free collagen organization measurements in thin histology sections.
  • Article
    Real-time polarization microscopy of fibrillar collagen in histopathology
    (Nature Research, 2021-09-24) Keikhosravi, Adib ; Shribak, Michael ; Conklin, Matthew W. ; Liu, Yuming ; Li, Bin ; Loeffler, Agnes ; Levenson, Richard ; Eliceiri, Kevin
    Over the past two decades, fibrillar collagen reorganization parameters such as the amount of collagen deposition, fiber angle and alignment have been widely explored in numerous studies. These parameters are now widely accepted as stromal biomarkers and linked to disease progression and survival time in several cancer types. Despite all these advances, there has not been a significant effort to make it possible for clinicians to explore these biomarkers without adding steps to the clinical workflow or by requiring high-cost imaging systems. In this paper, we evaluate previously described polychromatic polarization microscope (PPM) to visualize collagen fibers with an optically generated color representation of fiber orientation and alignment when inspecting the sample by a regular microscope with minor modifications. This system does not require stained slides, but is compatible with histological stains such as H&E. Consequently, it can be easily accommodated as part of regular pathology review of tissue slides, while providing clinically useful insight into stromal composition.