Roncalli Vittoria

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Last Name
Roncalli
First Name
Vittoria
ORCID
0000-0003-3024-7568

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  • Article
    Diapause vs. reproductive programs: transcriptional phenotypes in a keystone copepod
    (Nature Research, 2021-03-21) Lenz, Petra ; Roncalli, Vittoria ; Cieslak, Matthew C. ; Tarrant, Ann M. ; Castelfranco, Ann M. ; Hartline, Daniel K
    Many arthropods undergo a seasonal dormancy termed “diapause” to optimize timing of reproduction in highly seasonal environments. In the North Atlantic, the copepod Calanus finmarchicus completes one to three generations annually with some individuals maturing into adults, while others interrupt their development to enter diapause. It is unknown which, why and when individuals enter the diapause program. Transcriptomic data from copepods on known programs were analyzed using dimensionality reduction of gene expression and functional analyses to identify program-specific genes and biological processes. These analyses elucidated physiological differences and established protocols that distinguish between programs. Differences in gene expression were associated with maturation of individuals on the reproductive program, while those on the diapause program showed little change over time. Only two of six filters effectively separated copepods by developmental program. The first one included all genes annotated to RNA metabolism and this was confirmed using differential gene expression analysis. The second filter identified 54 differentially expressed genes that were consistently up-regulated in individuals on the diapause program in comparison with those on the reproductive program. Annotated to oogenesis, RNA metabolism and fatty acid biosynthesis, these genes are both indicators for diapause preparation and good candidates for functional studies.
  • Article
    Effects of petrogenic pollutants on North Atlantic and Arctic Calanus copepods: From molecular mechanisms to population impacts
    (Elsevier, 2023-12-28) Hansen, Bjorn Henrik ; Tarrant, Ann M. ; Lenz, Petra H. ; Roncalli, Vittoria ; Almeda, Rodrigo ; Broch, Ole Jacob ; Altin, Dag ; Tollefsen, Knut Erik
    Oil and gas industries in the Northern Atlantic Ocean have gradually moved closer to the Arctic areas, a process expected to be further facilitated by sea ice withdrawal caused by global warming. Copepods of the genus Calanus hold a key position in these cold-water food webs, providing an important energetic link between primary production and higher trophic levels. Due to their ecological importance, there is a concern about how accidental oil spills and produced water discharges may impact cold-water copepods. In this review, we summarize the current knowledge of the toxicity of petroleum on North Atlantic and Arctic Calanus copepods. We also review how recent development of high-quality transcriptomes from RNA-sequencing of copepods have identified genes regulating key biological processes, like molting, diapause and reproduction in Calanus copepods, to suggest linkages between exposure, molecular mechanisms and effects on higher levels of biological organization. We found that the available ecotoxicity threshold data for these copepods provide valuable information about their sensitivity to acute petrogenic exposures; however, there is still insufficient knowledge regarding underlying mechanisms of toxicity and the potential for long-term implications of relevance for copepod ecology and phenology. Copepod transcriptomics has expanded our understanding of how key biological processes are regulated in cold-water copepods. These advances can improve our understanding of how pollutants affect biological processes, and thus provide the basis for new knowledge frameworks spanning the effect continuum from molecular initiating events to adverse effects of regulatory relevance. Such efforts, guided by concepts such as adverse outcome pathways (AOPs), enable standardized and transparent characterization and evaluation of knowledge and identifies research gaps and priorities. This review suggests enhancing mechanistic understanding of exposure-effect relationships to better understand and link biomarker responses to adverse effects to improve risk assessments assessing ecological effects of pollutant mixtures, like crude oil, in Arctic areas.
  • Dataset
    RNA-Seq sample information and accessions numbers for the copepods Neocalanus flemingeri (Prince William Sound, Gulf of Alaska)(2015-2017) and Labidocera madurae (Kane‘ohe Bay, Oahu, Hawaii)(2017)
    (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-09-17) Hartline, Daniel K ; Lenz, Petra ; Hopcroft, Russell R. ; Roncalli, Vittoria ; Cieslak, Matthew C.
    This dataset includes accession information for RNA-seq data for the copepods Neocalanus flemingeri and Labidocera madurae used to generate de novo reference transcriptomes and for gene expression analysis. N. flemingeri adult females (CVI) were collected in Prince Williams Sound (Gulf of Alaska) during the fall (September 2015 and 2017) oceanographic cruises of the Seward line long-term observation program (ltop)(http://www.sfos.uaf.edu/sewardline/) and Northern Gulf of Alaska Long-Term Ecological Research Program (NGA LTER). For each sample collection date and preservation dates are listed. Labidocera madurae adult females (CVI) and mixed copepodid stages (CIII-CV) were collected in Kane‘ohe Bay, Oahu (Hawaii) in August 2015. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/821289