Stackhouse Bethany

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  • Article
    Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition
    (BioMed Central Ltd, 2010-12-08) Adey, Andrew ; Morrison, Hilary G. ; Asan ; Xun, Xu ; Kitzman, Jacob O. ; Turner, Emily H. ; Stackhouse, Bethany ; MacKenzie, Alexandra P. ; Caruccio, Nicholas C. ; Zhang, Xiuqing ; Shendure, Jay
    We characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously. We apply this method to sequencing a human genome and find that coverage biases are comparable to those of conventional protocols. We also extend its capabilities by developing protocols for sub-nanogram library construction, exome capture from 50 ng of input DNA, PCR-free and colony PCR library construction, and 96-plex sample indexing.