Lampert-Karako Sarit

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  • Article
    Chromatin dynamics and gene expression response to heat exposure in field-conditioned versus laboratory-cultured Nematostella vectensis
    (MDPI, 2021-07-12) Weizman, Eviatar N. ; Rinsky, Mieka ; Simon-Blecher, Noa ; Lampert-Karako, Sarit ; Yaron, Orly ; Tarrant, Ann M. ; Levy, Oren
    Organisms’ survival is associated with the ability to respond to natural or anthropogenic environmental stressors. Frequently, these responses involve changes in gene regulation and expression, consequently altering physiology, development, or behavior. Here, we present modifications in response to heat exposure that mimics extreme summertime field conditions of lab-cultured and field-conditioned Nematostella vectensis. Using ATAC-seq and RNA-seq data, we found that field-conditioned animals had a more concentrated reaction to short-term thermal stress, expressed as enrichment of the DNA repair mechanism pathway. By contrast, lab animals had a more diffuse reaction that involved a larger number of differentially expressed genes and enriched pathways, including amino acid metabolism. Our results demonstrate that pre-conditioning affects the ability to respond efficiently to heat exposure in terms of both chromatin accessibility and gene expression and reinforces the importance of experimentally addressing ecological questions in the field.
  • Article
    Identification of exceptionally potent adenosine deaminases RNA editors from high body temperature organisms
    (Public Library of Science, 2023-03-06) Avram-Shperling, Adi ; Kopel, Eli ; Twersky, Itamar ; Gabay, Orshay ; Ben-David, Amit ; Karako-Lampert, Sarit ; Rosenthal, Joshua J C ; Levanon, Erez Y ; Eisenberg, Eli ; Ben-Aroya, Shay
    The most abundant form of RNA editing in metazoa is the deamination of adenosines into inosines (A-to-I), catalyzed by ADAR enzymes. Inosines are read as guanosines by the translation machinery, and thus A-to-I may lead to protein recoding. The ability of ADARs to recode at the mRNA level makes them attractive therapeutic tools. Several approaches for Site-Directed RNA Editing (SDRE) are currently under development. A major challenge in this field is achieving high on-target editing efficiency, and thus it is of much interest to identify highly potent ADARs. To address this, we used the baker yeast Saccharomyces cerevisiae as an editing-naïve system. We exogenously expressed a range of heterologous ADARs and identified the hummingbird and primarily mallard-duck ADARs, which evolved at 40-42°C, as two exceptionally potent editors. ADARs bind to double-stranded RNA structures (dsRNAs), which in turn are temperature sensitive. Our results indicate that species evolved to live with higher core body temperatures have developed ADAR enzymes that target weaker dsRNA structures and would therefore be more effective than other ADARs. Further studies may use this approach to isolate additional ADARs with an editing profile of choice to meet specific requirements, thus broadening the applicability of SDRE.