All-optical mapping of barrel cortex circuits based on simultaneous voltage-sensitive dye imaging and channelrhodopsin-mediated photostimulation

View/ Open
Date
2015-03-31Author
Lo, Shun Qiang
Concept link
Koh, Dawn X. P.
Concept link
Sng, Judy C. G.
Concept link
Augustine, George J.
Concept link
Metadata
Show full item recordCitable URI
https://hdl.handle.net/1912/7386As published
https://doi.org/10.1117/1.NPh.2.2.021013DOI
10.1117/1.NPh.2.2.021013Abstract
We describe an experimental approach that uses light to both control and detect neuronal activity in mouse barrel cortex slices: blue light patterned by a digital micromirror array system allowed us to photostimulate specific layers and columns, while a red-shifted voltage-sensitive dye was used to map out large-scale circuit activity. We demonstrate that such all-optical mapping can interrogate various circuits in somatosensory cortex by sequentially activating different layers and columns. Further, mapping in slices from whisker-deprived mice demonstrated that chronic sensory deprivation did not significantly alter feedforward inhibition driven by layer 5 pyramidal neurons. Further development of voltage-sensitive optical probes should allow this all-optical mapping approach to become an important and high-throughput tool for mapping circuit interactions in the brain.
Description
© The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Neurophotonics 2 (2015): 021013, doi:10.1117/1.NPh.2.2.021013.
Collections
Suggested Citation
Neurophotonics 2 (2015): 021013The following license files are associated with this item: