All-optical mapping of barrel cortex circuits based on simultaneous voltage-sensitive dye imaging and channelrhodopsin-mediated photostimulation
All-optical mapping of barrel cortex circuits based on simultaneous voltage-sensitive dye imaging and channelrhodopsin-mediated photostimulation
Date
2015-03-31
Authors
Lo, Shun Qiang
Koh, Dawn X. P.
Sng, Judy C. G.
Augustine, George J.
Koh, Dawn X. P.
Sng, Judy C. G.
Augustine, George J.
Linked Authors
Alternative Title
Citable URI
As Published
Date Created
Location
DOI
10.1117/1.NPh.2.2.021013
Related Materials
Replaces
Replaced By
Keywords
Abstract
We describe an experimental approach that uses light to both control and detect neuronal activity in mouse barrel cortex slices: blue light patterned by a digital micromirror array system allowed us to photostimulate specific layers and columns, while a red-shifted voltage-sensitive dye was used to map out large-scale circuit activity. We demonstrate that such all-optical mapping can interrogate various circuits in somatosensory cortex by sequentially activating different layers and columns. Further, mapping in slices from whisker-deprived mice demonstrated that chronic sensory deprivation did not significantly alter feedforward inhibition driven by layer 5 pyramidal neurons. Further development of voltage-sensitive optical probes should allow this all-optical mapping approach to become an important and high-throughput tool for mapping circuit interactions in the brain.
Description
© The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Neurophotonics 2 (2015): 021013, doi:10.1117/1.NPh.2.2.021013.
Embargo Date
Citation
Neurophotonics 2 (2015): 021013