• Login
    About WHOAS
    View Item 
    •   WHOAS Home
    • Marine Biological Laboratory
    • Eugene Bell Center for Regenerative Biology and Tissue Engineering
    • Bell Center Data Sets
    • View Item
    •   WHOAS Home
    • Marine Biological Laboratory
    • Eugene Bell Center for Regenerative Biology and Tissue Engineering
    • Bell Center Data Sets
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of WHOASCommunities & CollectionsBy Issue DateAuthorsTitlesKeywordsThis CollectionBy Issue DateAuthorsTitlesKeywords

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Nematostella vectensis BAC sequences

    Thumbnail
    View/Open
    README file (1.880Kb)
    de_novo_assembly_mapped.zip (257.2Kb)
    raw_reads.zip (5.247Gb)
    mapped_raw_reads.zip (4.408Gb)
    de_novo_assembly.fastq.zip (532.6Mb)
    Date
    2013-07-11
    Author
    Fischer, Antje H. L.  Concept link
    Tulin, Sarah  Concept link
    Fredman, David  Concept link
    Smith, Joel  Concept link
    Metadata
    Show full item record
    Citable URI
    https://hdl.handle.net/1912/6068
    Related Material/Data
    https://hdl.handle.net/1912/6199
    DOI
    10.1575/1912/6068
    Keyword
     BAC; cnidarian; Illumina data 
    Abstract
    A key tool for investigating the regulation of genes is represented by Bacterial Artificial Chromosomes-reporter constructs (BAC). BACs are large insert libraries, often >>100 kb, which thus capture the genomic sequences surrounding a gene of interest, including all, or nearly all, of the elements underpinning regulation. Recombinant BACs, containing a reporter gene in place of the endogenous coding sequence of genes, can be utilized to drive the expression of reporter genes under the regulatory control of the gene of interest while still embedded within its genomic context. Systematic deletions within the BAC reporter construct can be used to identify the minimal reporter in an unbiased way, avoiding the risk of overlooking regulatory elements that may be many kilobases away from the transcription start-site. Nematostella vectensis (Edwardsiidae, Anthozoa, Cnidaria) has become an important model in regenerative biology, ecology, and especially in studies of evo-devo and gene-regulatory networks due to its interesting phylogenetic position and amenability to molecular techniques. This dataset represents the sequencing of 5 Nematostella vectensis BACs containing the genes of interest: NvDpp, NvChordin, NvFoxa, NvWnta, and NvWnt1.
    Description
    This dataset contains files described in the paper: Employing BAC-reporter constructs in the sea anemone Nematostella vectensis. The files are: raw reads, raw reads mapped to the genome, de novo assembled contigs, and the de novo assembled contigs mapped to the genome.
    Collections
    • Bell Center Data Sets
    Suggested Citation
    Dataset: Fischer, Antje H. L., Tulin, Sarah, Fredman, David, Smith, Joel, "Nematostella vectensis BAC sequences", 2013-07-11, DOI:10.1575/1912/6068, https://hdl.handle.net/1912/6068
     
    All Items in WHOAS are protected by original copyright, with all rights reserved, unless otherwise indicated. WHOAS also supports the use of the Creative Commons licenses for original content.
    A service of the MBLWHOI Library | About WHOAS
    Contact Us | Send Feedback | Privacy Policy
    Core Trust Logo