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dc.contributor.authorAuger, Helene  Concept link
dc.contributor.authorSasakura, Yasunori  Concept link
dc.contributor.authorJoly, Jean-Stephane  Concept link
dc.contributor.authorJeffery, William R.  Concept link
dc.date.accessioned2010-03-11T14:14:02Z
dc.date.available2010-03-11T14:14:02Z
dc.date.issued2009-12-06
dc.identifier.urihttps://hdl.handle.net/1912/3204
dc.descriptionAuthor Posting. © The Author(s), 2009. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Developmental Biology 339 (2010): 374-389, doi:10.1016/j.ydbio.2009.12.040.en_US
dc.description.abstractAscidians have powerful capacities for regeneration but the underlying mechanisms are poorly understood. Here we examine oral siphon regeneration in the solitary ascidian Ciona intestinalis. Following amputation, the oral siphon rapidly reforms oral pigment organs (OPO) at its distal margin prior to slower regeneration of proximal siphon parts. The early stages of oral siphon reformation include cell proliferation and re-growth of the siphon nerves, although the neural complex (adult brain and associated organs) is not required for regeneration. Young animals reform OPO more rapidly after amputation than old animals indicating that regeneration is age dependent. UV irradiation, microcautery, and cultured siphon explant experiments indicate that OPOs are replaced as independent units based on local differentiation of progenitor cells within the siphon, rather than by cell migration from a distant source in the body. The typical pattern of eight OPOs and siphon lobes is restored with fidelity after distal amputation of the oral siphon, but as many as sixteen OPOs and lobes can be reformed following proximal amputation near the siphon base. Thus, the pattern of OPO regeneration is determined by cues positioned along the proximal distal axis of the oral siphon. A model is presented in which columns of siphon tissue along the proximal-distal axis below pre-existing OPO are responsible for reproducing the normal OPO pattern during regeneration. This study reveals previously unknown principles of oral siphon and OPO regeneration that will be important for developing Ciona as a regeneration model in urochordates, which may be the closest living relatives of vertebrates.en_US
dc.description.sponsorshipThis research was supported by PhD fellowships from MRT and ARC to HA, Grants-in-Aid from MEXT, Japan, and the NIJ Cooperative Program (2008-B02) to YS, INRA, CNRS, the ANR Grant Choregnet, and the Marine Genomics Center of Excellence to J-SJ, Laura and Arthur Colwin and Frederick Bang Fellowships from the Marine Biological Laboratory, Woods Hole, MA to WRJ., and NSF grant (IBN-0611529) to WRJ.en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.relation.urihttps://doi.org/10.1016/j.ydbio.2009.12.040
dc.subjectAscidiansen_US
dc.subjectCionaen_US
dc.subjectTransgenic animalsen_US
dc.subjectRegenerationen_US
dc.subjectOral pigment organsen_US
dc.subjectPolarityen_US
dc.subjectPattern formationen_US
dc.titleRegeneration of oral siphon pigment organs in the ascidian Ciona intestinalisen_US
dc.typePreprinten_US


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