• Login
    About WHOAS
    View Item 
    •   WHOAS Home
    • MBLWHOI Library
    • Library Researchers
    • View Item
    •   WHOAS Home
    • MBLWHOI Library
    • Library Researchers
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of WHOASCommunities & CollectionsBy Issue DateAuthorsTitlesKeywordsThis CollectionBy Issue DateAuthorsTitlesKeywords

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Roles for Drosophila melanogaster myosin IB in maintenance of enterocyte brush-border structure and resistance to the bacterial pathogen Pseudomonas entomophila

    Thumbnail
    View/Open
    Article (4.843Mb)
    Figure 1: Myo1B localization in the adult optic lobe and photoreceptors (3.241Mb)
    Figure 2: Histology of whole mount, serial sectioned Myo1B heterozygous third instar larva (1.995Mb)
    Figure 3: Histology of whole mount, serial sectioned Myo1B homozygous third instar larva (2.602Mb)
    Date
    2007-09-12
    Author
    Hegan, Peter S.  Concept link
    Mermall, Valerie  Concept link
    Tilney, Lewis G.  Concept link
    Mooseker, Mark S.  Concept link
    Metadata
    Show full item record
    Citable URI
    https://hdl.handle.net/1912/1840
    As published
    https://doi.org/10.1091/mbc.E07-02-0191
    DOI
    10.1091/mbc.E07-02-0191
    Abstract
    Drosophila myosin IB (Myo1B) is one of two class I myosins in the Drosophila genome. In the larval and adult midgut enterocyte, Myo1B is present within the microvillus (MV) of the apical brush border (BB) where it forms lateral tethers between the MV membrane and underlying actin filament core. Expression of green fluorescent protein-Myo1B tail domain in the larval gut showed that the tail domain is sufficient for localization of Myo1B to the BB. A Myo1B deletion mutation exhibited normal larval gut physiology with respect to food uptake, clearance, and pH regulation. However, there is a threefold increase in terminal deoxynucleotidyl transferase dUTP nick-end labeling-positive enterocyte nuclei in the Myo1B mutant. Ultrastructural analysis of mutant midgut revealed many perturbations in the BB, including membrane tethering defects, MV vesiculation, and membrane shedding. The apical localization of both singed (fascin) and Dmoesin is impaired. BBs isolated from mutant and control midgut revealed that the loss of Myo1B causes the BB membrane and underlying cytoskeleton to become destabilized. Myo1B mutant larvae also exhibit enhanced sensitivity to oral infection by the bacterial pathogen Pseudomonas entomophila, and severe cytoskeletal defects are observed in the BB of proximal midgut epithelial cells soon after infection. Resistance to P. entomophila infection is restored in Myo1B mutant larvae expressing a Myo1B transgene. These results indicate that Myo1B may play a role in the local midgut response pathway of the Imd innate immune response to Gram-negative bacterial infection.
    Description
    Author Posting. © American Society for Cell Biology, 2007. This article is posted here by permission of American Society for Cell Biology for personal use, not for redistribution. The definitive version was published in Molecular Biology of the Cell 18 (2007): 4625-4636, doi:10.1091/mbc.E07-02-0191.
    Collections
    • Library Researchers
    Suggested Citation
    Molecular Biology of the Cell 18 (2007): 4625-4636
     
    All Items in WHOAS are protected by original copyright, with all rights reserved, unless otherwise indicated. WHOAS also supports the use of the Creative Commons licenses for original content.
    A service of the MBLWHOI Library | About WHOAS
    Contact Us | Send Feedback | Privacy Policy
    Core Trust Logo