Sparsened neuronal activity in an optogenetically activated olfactory glomerulus
Braubach, Oliver R.
Cohen, Lawrence B.
MetadataShow full item record
Glomeruli are the functional units of olfactory information processing but little remains known about their individual unit function. This is due to their widespread activation by odor stimuli. We expressed channelrhodopsin-2 in a single olfactory sensory neuron type, and used laser stimulation and simultaneous in vivo calcium imaging to study the responses of a single glomerulus to optogenetic stimulation. Calcium signals in the neuropil of this glomerulus were representative of the sensory input and nearly identical if evoked by intensity-matched odor and laser stimuli. However, significantly fewer glomerular layer interneurons and olfactory bulb output neurons (mitral cells) responded to optogenetic versus odor stimuli, resulting in a small and spatially compact optogenetic glomerular unit response. Temporal features of laser stimuli were represented with high fidelity in the neuropil of the glomerulus and the mitral cells, but not in interneurons. Increases in laser stimulus intensity were encoded by larger signal amplitudes in all compartments of the glomerulus, and by the recruitment of additional interneurons and mitral cells. No spatial expansion of the glomerular unit response was observed in response to stronger input stimuli. Our data are among the first descriptions of input-output transformations in a selectively activated olfactory glomerulus.
© The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Scientific Reports 8 (2018): 14955, doi:10.1038/s41598-018-33021-w.
Suggested CitationArticle: Braubach, Oliver R., Tombaz, Tuce, Geiller, Tristan, Homma, Ryota, Bozza, Thomas, Cohen, Lawrence B., Choi, Yunsook, "Sparsened neuronal activity in an optogenetically activated olfactory glomerulus", Scientific Reports 8 (2018): 14955, DOI:10.1038/s41598-018-33021-w, https://hdl.handle.net/1912/10640
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Perceptual stability during dramatic changes in olfactory bulb activation maps and dramatic declines in activation amplitudes Homma, Ryota; Cohen, Lawrence B.; Kosmidis, E. K.; Youngentob, S. L. (2007-03-30)We measured the concentration dependence of the ability of rats to identify odorants and compared these results with the calcium signals in the nerve terminals of the olfactory receptor neurons. Odorant identification ...
Xenobiotic monooxygenase activity and the response to inducers of cytochrome P-450 during embryonic and larval development in fish Binder, Robert L. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1981-08)Data demonstrating the presence and inducibility of the xenobiotic monooxygenase system in fish embryos and larvae are described. The ontogeny of benzo(a)pyrene monooxygenase (BPM) activity, and NADPH- and NADH-cytochrome ...
Identification of chemoautotrophic microorganisms from a diffuse flow hydrothermal vent at EPR 9° North using 13C DNA Stable Isotope Probing and Catalyzed Activated Reporter Deposition-Fluorescence in situ Hybridization Richberg, Kevin P. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2010-09)At deep‐sea hydrothermal vents chemolithoautotrophic microbes mediate the transfer of geothermal chemical energy to higher trophic levels. To better understand these underlying processes and the organisms catalyzing them, ...