Sparsened neuronal activity in an optogenetically activated olfactory glomerulus
Braubach, Oliver R.
Cohen, Lawrence B.
MetadataShow full item record
Glomeruli are the functional units of olfactory information processing but little remains known about their individual unit function. This is due to their widespread activation by odor stimuli. We expressed channelrhodopsin-2 in a single olfactory sensory neuron type, and used laser stimulation and simultaneous in vivo calcium imaging to study the responses of a single glomerulus to optogenetic stimulation. Calcium signals in the neuropil of this glomerulus were representative of the sensory input and nearly identical if evoked by intensity-matched odor and laser stimuli. However, significantly fewer glomerular layer interneurons and olfactory bulb output neurons (mitral cells) responded to optogenetic versus odor stimuli, resulting in a small and spatially compact optogenetic glomerular unit response. Temporal features of laser stimuli were represented with high fidelity in the neuropil of the glomerulus and the mitral cells, but not in interneurons. Increases in laser stimulus intensity were encoded by larger signal amplitudes in all compartments of the glomerulus, and by the recruitment of additional interneurons and mitral cells. No spatial expansion of the glomerular unit response was observed in response to stronger input stimuli. Our data are among the first descriptions of input-output transformations in a selectively activated olfactory glomerulus.
© The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Scientific Reports 8 (2018): 14955, doi:10.1038/s41598-018-33021-w.
Suggested CitationScientific Reports 8 (2018): 14955
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Perceptual stability during dramatic changes in olfactory bulb activation maps and dramatic declines in activation amplitudes Homma, Ryota; Cohen, Lawrence B.; Kosmidis, E. K.; Youngentob, S. L. (2007-03-30)We measured the concentration dependence of the ability of rats to identify odorants and compared these results with the calcium signals in the nerve terminals of the olfactory receptor neurons. Odorant identification ...
Subaqueous cryptodome eruption, hydrothermal activity and related seafloor morphologies on the andesitic North Su volcano Thal, Janis; Tivey, Maurice A.; Yoerger, Dana R.; Bach, Wolfgang (2016-04-28)North Su is a double-peaked active andesite submarine volcano located in the eastern Manus Basin of the Bismarck Sea that reaches a depth of 1154 m. It hosts a vigorous and varied hydrothermal system with black and white ...
Atmospheric forcing during active convection in the Labrador Sea and its impact on mixed-layer depth Schulze, Lena M.; Pickart, Robert S.; Moore, G. W. K. (John Wiley & Sons, 2016-09-22)Hydrographic data from the Labrador Sea collected in February–March 1997, together with atmospheric reanalysis fields, are used to explore relationships between the air-sea fluxes and the observed mixed-layer depths. The ...