Tide-related changes in mRNA abundance of aromatases and estrogen receptors in the ovary and brain of the threespot wrasse Halichoeres trimaculatus
Moon, Thomas W.
Vijayan, Mathilakath M.
MetadataShow full item record
The threespot wrasse (Halichoeres trimaculatus; Family Labridae) is a common coral reef species of the Indo-Pacific Ocean. Given that this species spawns daily at high tide (HT), we hypothesized that endocrine changes in relation to gonadal development are synchronized with the tidal cycle. To test this, we examined the transcript abundance of two cytochrome P450 aromatases (cyp19a and cyp19b) and two estrogen receptors (er and er) in the ovary and brain of this species in response to tidal change. When fish were collected around four tidal points [low tide (LT), flood tide (FT), high tide (HT), and ebb tide (ET)], gonadosomatic index and oocyte diameter increased around HT and FT, respectively. Ovulatory follicles were observed in ovaries around HT. Real-time quantitative polymerase-chain reaction revealed that mRNA abundance of cyp19a and er, but not er, in the ovary increased around ET and HT, respectively. On the other hand, mRNA levels of cyp19b in the forebrain were significantly higher around FT. Increases of er and er mRNA abundance around FT were observed in all areas of the brain and the midbrain, respectively. The changes in mRNA abundance of key genes involved in reproduction at specific tidal cycles, along with the development of the vitellogenic oocytes in the ovary, support our hypothesis that synchronization of endocrine changes to the tidal periodicity plays a role in the gonadal development of this species. We hypothesize that conversion of testosterone to E2 in the brain may be associated with the spawning behavior given that the wrasse exhibits group spawning with a territory-holding male around HT.
Author Posting. © The Author(s), 2018. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Ocean Science Journal 53 (2018): 239-249, doi:10.1007/s12601-018-0016-0.
Suggested CitationPreprint: Oh, Dae-Ju, Hur, Sung-Pyo, Bouchekioua, Selma, Takeuchi, Yuki, Udagawa, Shingo, Aluru, Neelakanteswar, Park, Yong-Ju, Park, Ji-Gweon, Kim, Se-Jae, Moon, Thomas W., Vijayan, Mathilakath M., Takemura, Akihiro, "Tide-related changes in mRNA abundance of aromatases and estrogen receptors in the ovary and brain of the threespot wrasse Halichoeres trimaculatus", 2018-05, https://doi.org/10.1007/s12601-018-0016-0, https://hdl.handle.net/1912/10504
Showing items related by title, author, creator and subject.
Griffith, David R. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2013-09)Steroidal estrogens are potent endocrine disrupting chemicals that are naturally excreted by vertebrates (e.g., humans and fish) and can enter natural waters through the discharge of treated and raw sewage. Because ...
Griffith, David R.; Wacker, Lukas; Gschwend, Philip M.; Eglinton, Timothy I. (2012-06)Steroids are potent hormones that are found in many environments. Yet, contributions from synthetic and endogenous sources are largely uncharacterized. The goal of this study was to evaluate whether carbon isotopes could ...
Callard, Gloria V.; Tarrant, Ann M.; Novillo, A.; Yacci, P.; Ciaccia, L.; Vajda, S.; Chuang, G.-Y.; Kozakov, D.; Greytak, S. R.; Sawyer, S.; Hoover, Cindi A.; Cotter, K. A. (2011-04-06)Classically, the estrogen signaling system has two core components: cytochrome P450 aromatase (CYP19), the enzyme complex that catalyzes the rate limiting step in estrogen biosynthesis; and estrogen receptors (ERs), ...