VitelloTag: A tool for high-throughput cargo delivery into oocytes
VitelloTag: A tool for high-throughput cargo delivery into oocytes
Date
2024-09-14
Authors
Clarke, D. Nathaniel
Kane, Akshay
Perillo, Margherita
Lowe, Christopher J.
Swartz, S. Zachary
Kane, Akshay
Perillo, Margherita
Lowe, Christopher J.
Swartz, S. Zachary
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DOI
10.1242/dev.202857
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Keywords
CRISPR/Cas9
Oocyte delivery
Vitellogenesis
Gene editing
Transgenesis
Oocyte delivery
Vitellogenesis
Gene editing
Transgenesis
Abstract
Delivering molecular tools into oocytes is essential for developmental and reproductive biology. Microinjection, the conventional method, is equipment intensive, often technically challenging and has a low yield, and is impractical in species with delicate oocytes or restricted spawning seasons. To overcome these limitations, we developed VitelloTag, a cost-effective, high-throughput system using vitellogenin-derived fusion proteins to enable efficient cargo delivery via receptor-mediated endocytosis. We demonstrate its utility by delivering Cas9/sgRNA complexes in two distantly related species for gene knockout.
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© The Author(s), 2024. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Clarke, D. N., Kane, A., Perillo, M., Lowe, C. J., & Swartz, S. Z. (2024). VitelloTag: A tool for high-throughput cargo delivery into oocytes. Development, dev.202857, https://doi.org/10.1242/dev.202857.
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Clarke, D. N., Kane, A., Perillo, M., Lowe, C. J., & Swartz, S. Z. (2024). VitelloTag: A tool for high-throughput cargo delivery into oocytes. Development, dev.202857.
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