In Vitro reconstitution and imaging of microtubule dynamics by fluorescence and label-free microscopy
In Vitro reconstitution and imaging of microtubule dynamics by fluorescence and label-free microscopy
Date
2020-11-24
Authors
Hirst, William G.
Kiefer, Christine
Abdosamadi, Mohammad Kazem
Schäffer, Erik
Reber, Simone
Kiefer, Christine
Abdosamadi, Mohammad Kazem
Schäffer, Erik
Reber, Simone
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10.1016/j.xpro.2020.100177
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Biophysics
Cell Biology
Microscopy
Cell Biology
Microscopy
Abstract
Dynamic microtubules are essential for many processes in the lives of eukaryotic cells. To study and understand the mechanisms of microtubule dynamics and regulation, in vitro reconstitution with purified components has proven a vital approach. Imaging microtubule dynamics can be instructive for a given species, isoform composition, or biochemical modification. Here, we describe two methods that visualize microtubule dynamics at high speed and high contrast: (1) total internal reflection fluorescence microscopy and (2) label-free interference reflection microscopy.
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© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Hirst, W. G., Kiefer, C., Abdosamadi, M. K., Schäffer, E., & Reber, S. In Vitro reconstitution and imaging of microtubule dynamics by fluorescence and label-free microscopy. STAR Protocols, 1(3), (2020): 100177, doi:10.1016/j.xpro.2020.100177.
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Hirst, W. G., Kiefer, C., Abdosamadi, M. K., Schäffer, E., & Reber, S. (2020). In Vitro reconstitution and imaging of microtubule dynamics by fluorescence and label-free microscopy. STAR Protocols, 1(3), 100177.
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