A revised evolutionary history of the CYP1A subfamily : gene duplication, gene conversion, and positive selection

dc.contributor.author Goldstone, Heather M. H.
dc.contributor.author Stegeman, John J.
dc.date.accessioned 2006-05-11T15:21:22Z
dc.date.available 2006-05-11T15:21:22Z
dc.date.issued 2005-12-31
dc.description Author Posting. © The Authors, 2005. This is the author's version of the work. It is posted here by permission of Springer for personal use, not for redistribution. The definitive version was published in Journal of Molecular Evolution 62 (2006): 708-717, doi:10.1007/s00239-005-0134-z. en
dc.description.abstract Members of cytochrome P450 subfamily 1A (CYP1As) are involved in detoxification and bioactivation of common environmental pollutants. Understanding the functional evolution of these genes is essential to predicting and interpreting species differences in sensitivity to toxicity by such chemicals. The CYP1A gene subfamily comprises a single ancestral representative in most fish species and two paralogs in higher vertebrates, including birds and mammals. Phylogenetic analysis of complete coding sequences suggests that mammalian and bird paralog pairs (CYP1A1/2 and CYP1A4/5, respectively) are the result of independent gene duplication events. However, comparison of vertebrate genome sequences revealed that CYP1A genes lie within an extended region of conserved fine-scale synteny, suggesting that avian and mammalian CYP1A paralogs share a common genomic history. Algorithms designed to detect recombination between nucleotide sequences indicate that gene conversion has homogenized most of the length of the chicken CYP1A genes, as well as the 5’ end of mammalian CYP1As. Together, these data indicate that avian and mammalian CYP1A paralog pairs resulted from a single gene duplication event and that extensive gene conversion is responsible for the exceptionally high degree of sequence similarity between CYP1A4 and CYP1A5. Elevated non-synonymous/synonymous substitution ratios within a putatively unconverted stretch of ~250 bp suggests that positive selection may have reduced the effective rate of gene conversion in this region, which contains two substrate recognition sites. This work significantly alters our understanding of functional evolution in the CYP1A subfamily, suggesting that gene conversion and positive selection have been the dominant processes of sequence evolution. en
dc.description.sponsorship Funding for this work was provided by the NIH Superfund Basic Research Program at Boston University (5-P42-ES-07381) and by the Woods Hole Oceanographic Institution. en
dc.format.extent 12014 bytes
dc.format.extent 431144 bytes
dc.format.mimetype text/plain
dc.format.mimetype application/pdf
dc.identifier.uri https://hdl.handle.net/1912/927
dc.language.iso en_US en
dc.relation.uri https://doi.org/10.1007/s00239-005-0134-z
dc.subject Cytochrome P450 en
dc.subject Gene conversion en
dc.subject Gene duplication en
dc.subject Chicken en
dc.subject Mammalian en
dc.title A revised evolutionary history of the CYP1A subfamily : gene duplication, gene conversion, and positive selection en
dc.type Preprint en
dspace.entity.type Publication
relation.isAuthorOfPublication c3bc4ff4-7b40-4a4a-a2fc-3b70d6227644
relation.isAuthorOfPublication 07abf0ef-ea12-4778-81c9-115ca35f9b1e
relation.isAuthorOfPublication.latestForDiscovery c3bc4ff4-7b40-4a4a-a2fc-3b70d6227644
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
421.04 KB
Adobe Portable Document Format
Author's final draft
No Thumbnail Available
11.73 KB
Plain Text
Supplementary material: The alignment of CYP1A coding sequences used for all analyses presented herein is provided in multi-sequence FASTA format (vertebrate_CYP1As.fasta).
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
1.97 KB
Item-specific license agreed upon to submission