SAG-RAD: a method for single-cell population genomics of unicellular eukaryotes
SAG-RAD: a method for single-cell population genomics of unicellular eukaryotes
Date
2023-04-20
Authors
Gollnisch, Raphael
Wallenius, Joel
Gribble, Kristin E.
Ahrén, Dag
Rengefors, Karin
Wallenius, Joel
Gribble, Kristin E.
Ahrén, Dag
Rengefors, Karin
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DOI
10.1093/molbev/msad095
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Keywords
Culturing bias
Microbial genetic diversity
Microeukaryotes
Restriction-site associated DNA (RAD) Sequencing
Single amplified genome (SAG)
Whole-genome amplification (WGA)
Microbial genetic diversity
Microeukaryotes
Restriction-site associated DNA (RAD) Sequencing
Single amplified genome (SAG)
Whole-genome amplification (WGA)
Abstract
Sequencing of reduced representation libraries enables genotyping of many individuals for population genomic studies. However, high amounts of DNA are required, and the method cannot be applied directly on single cells, preventing its use on most microbes. We developed and implemented the analysis of single amplified genomes followed by restriction-site-associated DNA sequencing to bypass labor-intensive culturing and to avoid culturing bias in population genomic studies of unicellular eukaryotes. This method thus opens the way for addressing important questions about the genetic diversity, gene flow, adaptation, dispersal, and biogeography of hitherto unexplored species.
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© The Author(s), 2023. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Gollnisch, R., Wallenius, J., Gribble, K., Ahrén, D., & Rengefors, K. SAG-RAD: a method for single-cell population genomics of unicellular eukaryotes. Molecular Biology and Evolution, 40(5), (2023): msad095, https://doi.org/10.1093/molbev/msad095.
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Gollnisch, R., Wallenius, J., Gribble, K., Ahrén, D., & Rengefors, K. (2023). SAG-RAD: a method for single-cell population genomics of unicellular eukaryotes. Molecular Biology and Evolution, 40(5), msad095.