Effects of an allelochemical in Phaeodactylum tricornutum filtrate on Heterosigma akashiwo : morphological, physiological and growth effects
Effects of an allelochemical in Phaeodactylum tricornutum filtrate on Heterosigma akashiwo : morphological, physiological and growth effects
Date
2017-08
Authors
Wang, Rui
Xue, Qiaona
Wang, Jiangtao
Tan, Liju
Zhang, Qingchun
Zhao, Yue
Anderson, Donald M.
Xue, Qiaona
Wang, Jiangtao
Tan, Liju
Zhang, Qingchun
Zhao, Yue
Anderson, Donald M.
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Person
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Keywords
Allelochemical
Phaeodactylum tricornutum
Heterosigma akashiwo
Flow cytometry
Physiological characters
Phaeodactylum tricornutum
Heterosigma akashiwo
Flow cytometry
Physiological characters
Abstract
The effects of an allelochemical extracted from the culture filtrate of diatom Phaeodactylum
tricornutum on the raphidophyte Heterosigma akashiwo were investigated using a series of
morphological, physiological and biochemical characters. Growth experiments showed that H.
akashiwo was significantly inhibited immediately after exposure to the allelochemical, with many cells
rapidly dying and lysing based on microscopic observation. The effects of the allelochemical on the
surviving cells were explored using Scanning Electron Microscopy (SEM) and Flow cytometry (FCM),
the latter by examination of a suite of physiological parameters (membrane integrity, esterase activity,
chlorophyll-a content, membrane potential). The results demonstrate that the membrane of H. akashiwo
was attacked by the allelochemical directly, causing cell membrane breakage and loss of integrity.
Esterase activity was the most sensitive indicator of the impacts of the allelochemical. Membrane potential and chlorophyll-a content both showed significant decreases following exposure of the
Heterosigma cells to high concentrations of the allelochemical for 5 and 6 days. Both were affected,
but the membrane potential response was more gradual compared to other effects. The cell size of H.
akashiwo did not change compared with the control group. The surviving cells were able to continue to
grow and in a few days, re-establish a successful culture, even in the presence of residual
allelochemical, suggesting either development of cellular resistance, or the degradation of the chemical.
Description
© The Author(s), 2017. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Chemosphere 186 (2017): 527-534, doi:10.1016/j.chemosphere.2017.08.024.